Ink Chromatography Experiment. Safety and First Aid. This section will only deal with the specific safety issues regarding the chemicals involved and ...
Chromatography is a method for analyzing mixtures by separating them into the chemicals from which they are made. It can be used to separate mixtures like ink, blood, gasoline, and lipstick. In ink chromatography, you are separating the colored pigme
Forensics: Ink Chromatography Lab 2015 Background: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which they are made
chosen for this experiment because when the eluting solution reaches that line, any inks that are mixtures should be clearly separated. When the solvent front reaches the finish line, the paper should be removed immediately from contact with the mobi
SCIENCE EXPERIMENTS ON FILE ™ Revised Edition. 5.31-1. Using Paper Chromatography to Separate Ink. Virgil A. Sestini. Topic. Paper chromatography .... each experiment. If you are working in a lab or in the field, do not work alone. This book assumes
Instructor guide and answer key. Materials: ... others. If this lab is done in the winter when leaves are not available, spinach leaves can be used. ... 1. Experiment with different types of leaves. Which leaf has the greatest amount of pigment? 2. P
The purpose of this experiment is to use liquid chromatography to separate the component ... 2 Procedure There are two parts to this lab. In part one,
High-Performance Liquid Chromatography High Performance Liquid Chromatography (HPLC) was developed in the late 1960s and early 1970s. Today it is widely applied for
paper and some are more attracted to the alcohol or water. In this experiment you learn how to prepare chromatography paper and solution. You will also use these skills to separate your own samples of inks into their basic colors. In this activity we
Download This document contains a more detailed description of the Chemistry in the Olympics Global. Experiment. It is designed to look at the effects of sports drinks on physical performance. The experiment involves running 100 metres with or
Centrifugal pumps – pump curves. • Real pumps are never 'ideal' and the performance of the pumps are determined experimentally by the manufacturer and typically given in terms of graphs or pump curves. • Typically performance is given by curves of: •
contain rings, of course, but for which classes can the functional group itself be part of a ring? For example, the –O– of an ether ... of different compounds and determine what key absorptions are characteristic of each functional group or can be ..
Download such as economic theory, experimental economics, psychophysiology, and lie- detection. Therefore, we use this methodology appendix to address issues that might already be very familiar to some readers, but not to the rest. In particular,
applications of chromatography – identification of an unknown ink sample and the separation ... Calculating Rf values and interpreting the results. In order to obtain a quantitative measure of the extent of movement of a component in a paper chromato
Download such as economic theory, experimental economics, psychophysiology, and lie- detection. Therefore, we use this methodology appendix to address issues that might already be very familiar to some readers, but not to the rest. In particular,
Download This document contains a more detailed description of the Chemistry in the Olympics Global. Experiment. It is designed to look at the effects of sports drinks on physical performance. The experiment involves running 100 metres with or
1 EXPERIMENT 12: Empirical Formula of a Compound INTRODUCTION Chemical formulas indicate the composition of compounds. A formula that gives only the simplest ratio
CHAPTER 11: REFLECTIONS ON THE STANFORD PRISON EXPERIMENT: GENESIS, TRANSFORMATIONS, CONSEQUENCES Philip G. Zimbardo Stanford University Christina Maslach
Hans F. K. Günther, Rassenkunde des deutschen Volkes, München 1937, S. 109, ... Kleine Rassenkunde Europas, München 1925, S. 64, „haben so viele Merkmale
Download [42] Lensmeyer GL, Onsager C, Carlson IH and Wiebe DA. (1995) Journal of Chromatography A 691: 239}246. [43] LeBelle M, Pike RK, Graham SJ, Ormsby ED and. Bogard H (1996) Journal of Pharmaceutical and Bio- medical Analysis 14: 793}800.
Spinach Chromatography Lab ... Chromatography is a technique for separating and identifying substances in a ... Answers should be in complete
In paper chromatography, the sample mixture is applied to a piece of filter paper, the edge of the paper is immersed in a ... a component in a paper chromatography experiment, we can calculate an "Rf value" for each separated ... All of the solutions
Download PO-CON1575E. On-line supercritical fluid extraction/ supercritical fluid chromatography: A novel approach to the cleaning validation for pharmaceutical manufacturing. HPLC 2015 PSA-SFC-14. Daisuke Nakayama, Hidetoshi Terada, Minori Nak
Ink Chromatography Experiment Safety and First Aid This section will only deal with the specific safety issues regarding the chemicals involved and used in the video; other safety data may be required depending on the chemicals used. There are likely to be other safety issues that will need to be addressed outside the scope of this section and a full risk assessment should be performed prior to undertaking the experiment.
Students should not ingest any of the experiment; good lab practice starts young!
Rubbing alcohol is toxic if ingested and flammable. Avoid prolonged inhalation of fumes.
First Aid – wash the affected area with water. If rubbing alcohol is ingested, DO NOT induce vomiting; rinse mouth with water and seek medical assistance.
Glossary
Chromatography – a set of laboratory techniques used for the separation of mixtures
Capillary action – the ability of a chemical to flow into narrow spaces without the assistance of an external force, often against the force of gravity
Suggested Teaching Points
Analytical science – this experiment will introduce students to analytical science by investigating the chemical composition of inks
Chromatography – this experiment will introduce a simple method for chromatography
Investigative technique – this experiment will allow students to compare the chemical composition of different inks
Problem solving and data interpretation – please see the ‘Murder Mystery’ section of the “Suggested improvements / student challenge” section
What to Expect Please see the video for a detailed depiction of the experiment. In this experiment, we will be investigating the different chemical compositions of a selection of inks. In order to achieve this, we will use an analytical technique, known as chromatography, to separate out the different mixtures of chemicals present in the ink. For this experiment, filter paper and a solvent (either water or rubbing alcohol in our case) will separate out the chemicals.
A small sample of the ink to be investigated is placed on the bottom of the filter paper and the solvent allowed to be drawn through the filter paper via capillary action. As the solvent passes the ink, any chemicals that dissolve in the solvent will be carried along as the solvent continues up the filter paper, while insoluble chemicals will remain at the bottom. The distance that each separate chemical travels up the filter paper is dependent on how well it dissolves in the solvent and how strongly it binds to the filter paper. For a chemical that dissolves well in the solvent and does not bind to the filter paper, it will travel much further than a chemical that dissolves well but binds strongly to the filter paper. Once the solvent has reached the top of the filter paper, each ink will leave behind a specific characteristic pattern showing the mixture of chemicals that were contained in the ink.
Equipment and Chemicals
Water
Rubbing alcohol
Filter paper
Pencil
Selection of different inks
Supplementary Instructions
The baseline must be drawn in pencil. If you use a pen, the ink may move and mix with the inks you are investigating.
It is essential that the level of solvent is below the pencil baseline and does come into contact with any of the inks you are investigating.
To increase the rate at which the solvent travels through the filter paper, cover the top of the container to make an airtight seal.
Try and handle the filter paper as little as possible while wet to prevent tearing.
Suggested Questions for Students
What use could chromatography have in chemical industry? – chromatography is an essential technique utilised by a wide of analytical professions. For example, synthetic chemists use chromatography to purify chemicals before they are allowed into use (e.g. pharmaceutical drugs). Other uses include forensic science and checking the purity of water.
Why do inks have different coloured chemicals in them? – inks tend to have a variety of different coloured dyes to give the desired overall colour. This is demonstrated in the video showing that green highlighters are made of a blue and yellow dye.
Suggested Improvements / Student Challenge
Use other solvents – students could investigate how other solvents separate out the different chemicals present in ink. Ethyl acetate and acetone (found in different types of nail varnish remover; see the ingredients label for which) could be used; remember to complete a new risk assessment for this!
Murder mystery – a scenario could be set up in which students undertake the role of a forensic scientist. The students could be given a sample of ink and told that this was found on the ‘victim’. The students should then be given a selection of other inks, which provide unique dye patterns when separated, that have been obtained from the ‘suspects’. The students would then compare the ‘victim’ sample with those of the ‘suspects’ to discover who was responsible.
Clean up and Waste Disposal The specific waste disposal protocols for this experiment will depend upon the choice of liquids used. Spillages can be cleaned with water and paper towel. Filter paper can be disposed of in the bin.
