PRODUCT BULLETIN Microbial Identification
MicroSeq® Microbial Identification System
Link to Microbial ID, offering low-cost DNA Sequencing by the MicroSeq System
• Revolutionary phylogenetic system provides accurate, reproducible results • Elimination of special growth requirements and preliminary testing for easier use • Rapid identification of difficult samples saves valuable staff time • Rigorous validation of sequence libraries ensures accurate species identification • Innovative software allows users to create and search custom sequence libraries Unmatched Standard for Microbial Identification The MicroSeq® Microbial Identification System is a complete solution for all eubacteria, yeasts, and filamentous fungi. It rapidly identifies slow-growers and non-fermenters (in less than 24 hours) that are often impossible to identify with conventional systems. No prior knowledge of the organism is required since the system uses universal, domain-level primers. Accurate Results A phylogenetic approach based on ribosomal RNA (rRNA) gene sequences makes the MicroSeq system the most accurate microbial identification system available. In fact, the latest edition of Bergey’s Manual of Systematic Bacteriology has been reorganized to reflect these advances in bacterial identification. Objective, Reproducible Identification Method The MicroSeq system eliminates subjective phenotypic or growth-based test interpretation since it is based on
Electropherogram from an ABI PRISM® 3100 Genetic Analyzer of unidentified filamentous fungi isolate.
rRNA gene sequences. Results are reproducible across laboratories and over time, and because the system employs the PCR process, culture conditions do not influence results. Comprehensive and Customizable Libraries Comprehensive sequence libraries contain a vast range of bacterial and fungal species relevant to the branches of applied microbiology in which microbial control and the elimination of contamination are critical. With the flexible MicroSeq software you can create customized libraries for specific sequences of interest derived from proprietary strains.
following a rigorous validation process to ensure accurate species identification. The MicroSeq libraries contain correctly identified organisms and quality controlled sequences unlike other widely available databases. MicroSeq Systems
Library Contents
MicroSeq 16S rDNA Bacterial full-gene Bacterial Identification and 500 bp libraries System 1,400+ validated entries Includes extensive coverage of gram-negative nonfermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. ®
MicroSeq® D2 LSU rDNA Fungal Identification System
Contains 900 entries on yeasts and filamentous fungi.
Quality-Controlled Libraries Applied Biosystems constructed the MicroSeq libraries using type strains from pre-eminent culture collections
PRODUCT BULLETIN
Broad Applicability The MicroSeq system contains sequence libraries for a broad range of organisms found in many fields of applied microbiology. The Whole Product Solution Applied Biosystems has combined state-of-the-art instruments, reagents, and software to provide your laboratory with the most advanced, comprehensive system available for accurate microbial identification. All of our products undergo rigorous quality testing to ensure that they meet our performance specifications. The system is supported by our extensive worldwide service organization. For more information, please contact your Applied Biosystems sales representative or your local sales office. Flowchart of the MicroSeq identification process. DNA is extracted in a simple 10-minute procedure with the PrepMan™ Ultra DNA extraction reagent. The DNA is then amplified by PCR and cycle sequenced with easy-to-use master mixes provided in the MicroSeq kits. Samples can be electrophoresed on any ABI PRISM genetic analyzer. Resulting sample files are analyzed and compared to our comprehensive sequence libraries for identification of the unknown sample.
Research Specialty/Industry
Applications
Pharmaceutical
Environmental monitoring of clean-production areas Quality-control testing of raw materials and end-product sterility Fermentation monitoring Natural-product drug discovery
Clinical Research Labs
Identification of slow growers, non-fermenters, and fall-through organisms
Culture-Collection Centers
Culture authentication De-replication
Environmental
Monitoring the biodiversity of natural populations Bioremediation
Agricultural
Identification of plant pathogenic fungi in crops and storage facilities Monitoring of fungi in crop-growing regions
Food and Beverage
Environmental-contamination monitoring Process control of fermented products Tracking of spoilage organisms from outbreaks
www.appliedbiosystems.com
PCR Service Rights Applied Biosystems MicroSeq Microbial Identification kits carry a label license that conveys certain PCR rights for bacterial and fungal identification testing as a commercial service when used in conjunction with an Authorized Thermal Cycler. No additional license or royalty payments with respect to PCR service rights are required.* *Other licenses may be required for your specific application.
Assembly Tools Easy assembly of sequences derived from ABI PRISM® sample files created by ABI PRISM® genetic analyzers.
Tree Tools Construction of trees (dendograms) that enable phylogenetic analysis and similarity comparisons (Neighbor Joining, UPGMA, WPGMA).
BLAST and Full-Search Tools Microbial identification provided by sequence searches of MicroSeq® databases using a variety of search algorithms such as BLAST and Needleman Wunsch.
Align and Concise Align Tools Alignments of sequence data with two viewing options: Full Gene and Concise.
MicroSeq® Libraries Includes bacterial full gene and 500 bp libraries with over 1,400 validated entries, and a yeast and filamentous fungi library with approximately 900 validated entries.
The MicroSeq® Analysis Software provides drag-and-drop simplicity to aid in the analysis of sequences obtained with MicroSeq reagent kits. MicroSeq software contains a complete sequence analysis package that includes: Assembly Tools, Tree Tools, BLAST and Full-Search Tools, Align and Concise Align Tools, and the MicroSeq Libraries.
www.appliedbiosystems.com
Ordering Information Description
Part Number
The MicroSeq D2 LSU rDNA Fungal Identification Kit provides all of the reagents for sequencing the D2 expansion segment region of the nuclear large-subunit (LSU) rRNA gene.
MicroSeq D2 LSU rDNA Fungal Identification System 4311768 Includes PCR and sequencing modules (sufficient for 50 identifications), protocol, and quick start card MicroSeq D2 LSU rDNA Fungal Identification Kit Includes PCR and sequencing modules (sufficient for 50 identifications)
4311769
MicroSeq D2 LSU rDNA Fungal Identification System Protocol
4312442
MicroSeq D2 LSU rDNA Fungal Identification System Quick Start Card
4312443
The MicroSeq 500 16S rDNA Bacterial Sequencing Kit provides all the reagents for analysis of the first 500 base pairs of the 16S rDNA gene.
Worldwide Sales Offices
MicroSeq 500 16S rDNA Bacterial Identification System 4307233 Includes PCR and sequencing modules (sufficient for 50 identifications), protocol, and quick start card
Applied Biosystems vast distribution and service network, composed of highly trained support and applications personnel, reaches into 150 countries on six continents. For international office locations, please call the division headquarters or refer to our Web site at www.appliedbiosystems.com.
MicroSeq 500 16S rDNA Bacterial Identification Kit Includes PCR and sequencing modules (sufficient for 50 identifications)
4306775
MicroSeq 500 16S rDNA Bacterial Identification System Protocol
4307388
MicroSeq 500 16S rDNA Bacterial Identification System Quick Start Card
4308132
The MicroSeq Full Gene 16S rDNA Bacterial Sequencing Kit provides all of the reagents for sequencing the entire 16S rRNA bacterial gene.
MicroSeq Full Gene 16S rDNA Bacterial Identification System 4303300 Includes PCR and sequencing modules (sufficient for 10 identifications), protocol, and quick start card MicroSeq Full Gene 16S rDNA Bacterial Identification Kit Includes PCR and sequencing modules (sufficient for 10 identifications)
Applera Corporation is committed to providing the world's leading technology and information for life scientists. Applera Corporation consists of the Applied Biosystems and Celera Genomics businesses.
403085 Headquarters
MicroSeq Full Gene 16S rDNA Bacterial Identification System Protocol
4303233
MicroSeq Full Gene 16S rDNA Bacterial Identification System Quick Start Card
4303235
PrepMan™ Ultra Sample Preparation Reagent Includes reagents (sufficient for 100 preparations), protocol, and quick start card
4322547
PrepMan Ultra Sample Preparation Reagent 100 preparations
4318930
850 Lincoln Centre Drive Foster City, CA 94404 USA Phone: 650.638.5800 Toll Free: 800.345.5224 Fax: 650.638.5884 For Research Use Only. Not for use in diagnostic procedures.
MicroSeq® Analysis Software (Requires Macintosh® computer [OS: 7.5 to 9.0.4], 256 MB RAM, and 350 MHz processor)
The PCR process is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. ABI PRISM, Applied Biosystems, and MicroSeq are registered trademarks, and AB (Design), Applera, and PrepMan are trademarks of Applera Corporation or its subsidiaries in the US and certain other countries. Macintosh is a registered trademark of Apple Computer, Inc. All other trademarks are properties of their respective owners.
MicroSeq Analysis Software Software only, no library access
4306753C
MicroSeq Analysis Software Includes hardware key for 100 MicroSeq library searches
4306755C
MicroSeq Analysis Software Includes hardware key for 1,000 MicroSeq library searches
4306756C
MicroSeq Analysis Software Includes hardware key for 2,500 MicroSeq library searches
4306757C
MicroSeq Analysis Software Includes hardware key for unlimited MicroSeq library searches
4306752C
©2001 Applied Biosystems. All rights reserved. Information subject to change without notice.
MicroSeq D2 Fungal Database
4319240C
Printed in the USA, 7/2001, JPI Publication 116PB05-02
www.appliedbiosystems.com
Completion of an RUO Certificate is required prior to the initial purchase of the MicroSeq kits. The RUO Certificate acknowledges that the MicroSeq kits are being purchased for research use only, and that the information obtained from the MicroSeq system will not be used for the diagnostic purpose of any patient care decision or for the purpose of transmitting information to patients.
