Plants contain chlorophyll, a green pigment, as well as carotenoids, pigments that range in color from red to orange to yellow. Question: Do green lea...
Spinach Chromatography Lab ... Chromatography is a technique for separating and identifying substances in a ... Answers should be in complete
applications of chromatography – identification of an unknown ink sample and the separation ... Calculating Rf values and interpreting the results. In order to obtain a quantitative measure of the extent of movement of a component in a paper chromato
Instructor guide and answer key. Materials: ... others. If this lab is done in the winter when leaves are not available, spinach leaves can be used. ... 1. Experiment with different types of leaves. Which leaf has the greatest amount of pigment? 2. P
In paper chromatography, the sample mixture is applied to a piece of filter paper, the edge of the paper is immersed in a ... a component in a paper chromatography experiment, we can calculate an "Rf value" for each separated ... All of the solutions
Paper Chromatography Lab Chromatography is a method for analyzing complex mixtures by separating them into the chemicals from which they are made
Paper Chromatography Lab ... 4th – Have your answers checked by the teacher. Keep trying until you are able to identify all 6 pens! Pen A matches # _____
Lab Activity H5 Paper Chromatography of M&M’s OUTCOMES After completing this lab activity, ... Considering your answers above, how many dyes,
paper and some are more attracted to the alcohol or water. In this experiment you learn how to prepare chromatography paper and solution. You will also use these skills to separate your own samples of inks into their basic colors. In this activity we
SEPARATION OF DYES BY PAPER CHROMATOGRAPHY LAB. Purpose: Like all chemicals, ... solution. 4. Paper chromatography—a method chemists use to separate one chemical from another using their differences in physical properties . Paper chromatography has s
Chromatography Lab activity — Worksheet 1 Chromatography Lab Worksheet Chromatography is a way to look at complex mixtures by separating them into their
Forensics: Ink Chromatography Lab 2015 Background: Chromatography is a method for analyzing mixtures by separating them into the chemicals from which they are made
The key to lab-tips of ... or not a student has changed his or her answers after a test has been graded and returned? Title: A Lab on Chromatography Inks
Questions and Conclusions. 1. What criteria were used to identify the ion(s) found in your unknown? Explain your answer in at least three sentences. Include any difficulties in identifying any ions. 2. If you let the experiment run for only 30 minute
Paper Chromatography Lab Questions. Answer each of the following questions. Base your answers (conclusions) on reasonable interpretations of your data and provide justification. 1. Why is it important to keep the spots applied to the chromatography p
In general, chromatography involves two phases: • A Stationary Phase. This is typically a solid. In today's lab, it will be paper. • A Moving Phase. This is typically a liquid. In today's lab, it will be a very dilute solution of salt in water. In ge
chosen for this experiment because when the eluting solution reaches that line, any inks that are mixtures should be clearly separated. When the solvent front reaches the finish line, the paper should be removed immediately from contact with the mobi
In a previous experiment you have seen how differences in the polarity of molecules can ... The point of this experiment is not to develop a wash-day solution to all ... In paper chromatography substances are applied to a piece of absorbent paper. A
Jan 31, 1996 ... measures the absorption of light by a solution at any wavelength(s) selected by the experimenter. Light visible ... To separate pigments from leaves of a green plant using paper chromatography and to determine .... 180 mL petroleum e
Fisher in rolls of 100 yards (300 feet). Since students will be working in groups of 3 or 4, you will need to cut 30-32 strips of chromatography paper, one for each student. 2. Obtain brands of ink that will yield good results. Pens that work well in
Plant Traveling Lab. TTU/HHMI at CISER. 2010 2 The bands derived in paper chromatography contain the pigments found in the plant. The bands can be cut apart, and
and/or tape your chromatogram into the lab book. • Data table: Determine the Rf values for each component in each ink. Calculations and Data Analysis: 1. Calculate Rf values for each ink or dye using the formula. Rf. = distance traveled by the compou
Oct 4, 2008 ... Plant leaves contain a number of important pigments including chlorophylls, carotenes, and xanthophylls. During ... During this lab you will have a chance to collect leaves of various colors and analyze the different ... Thin-layer ch
Honors Biology: Chromatography Lab Answer Key Terms and Definitions (1 Point Each): 1. Chromatogram 2. Mobile Phase 3. Stationary Phase 4. Solute
www.homebaking.org A BAKER’S DOEN Lab A Bankers Pantry of Resources 149 Resources and References: For Teacher: The following textbook baking resources are standards
Chromatography of Spinach.08.doc
Paper Chromatography of a Spinach Leaf Lab Background Information: Paper chromatography is a process that uses special filter paper to separate and identify the different substances in a mixture. Chromatography means “to write with color.” The substances in the mixture dissolve in the alcohol and move up the paper. The heavier substances move up the paper more slowly. The lighter substances move up the paper more quickly. So heavy and light substances get separated from one another on the paper. Plants contain chlorophyll, a green pigment, as well as carotenoids, pigments that range in color from red to orange to yellow. Question: Do green leaves also contain other pigments? Hypothesis: Record on the Analysis Sheet. Materials: Isopropyl alcohol spinach leaf chromatography paper paperclip beaker
ruler scissors pencil tape colored pencils
Procedure: 1. Obtain a strip of chromatography paper. 2. Use a ruler to measure and draw a light pencil line 2-cm above the bottom of the paper strip. 3. Here is the tricky part! Place the edge of the spinach leaf over the pencil line and using the edge of a coin gently press on the spinach leaf to create a single green line over the pencil line. You want this line to be thin and concentrated with the pigment from the spinach leaf. Therefore, repeat this edging process carefully about 3-4 times. Be sure not to press too hard or you will poke a hole through the paper. 4. Tape the top of the paper strip to a pencil so that the end of the strip with the green line hangs down. The pencil should be able to sit across the top of the beaker with the bottom of the paper strip just touching the bottom of the beaker. Cut off any excess paper from the TOP of the strip if it is too long. 5. Remove the pencil/paper strip contraption from the beaker for the moment. Record observations in data table. 6. Carefully add isopropyl alcohol to the beaker until it reaches a depth of 1-cm in the beaker.
Chromatography of Spinach.08.doc 7. Lay the pencil across the top of the beaker with the paper strip extending into the alcohol. MAKE SURE THAT THE LEVEL OF THE ALCOHOL IS BELOW THE GREEN LINE ON YOUR PAPER STRIP! IF THE ALCOHOL IS GOING TO COVER THE GREEN LINE, POUR OUT SOME ALCOHOL BEFORE YOU GET THE GREEN LINE WET! 8. Observe as the alcohol gets absorbed and travels up the paper by capillary action. This may take up to 20 minutes. Do not touch your experiment during this time. 9. When the alcohol has absorbed to approximately 1-cm below the pencil, you may remove the pencil/paper strip from the beaker to dry on your counter. With a pencil, mark the distance the alcohol has traveled on the paper, as well as the distance each pigment has traveled. 10. Using colored pencils, draw your results in the data table. 11. Using a ruler and the following formula, measure the Rf values of each pigment. Since the fastest molecules will travel the greatest distance, or to the highest point along the strip, the relative distances can be measured, and the flow rate (migration) of the molecules (Rf) can be calculated by using the following formula: Rf = Distance pigment traveled Distance solvent traveled
Chromatography of Spinach.08.doc Name: ________________________________________________ Date: ________________ Block: ____ Paper Chromatography of a Spinach Leaf Lab – ANALYSIS SHEET Pre-lab: Question: Do green leaves also contain other pigments? Hypothesis:
Data: Filter Paper
Use colored pencils to draw your observations
Before Paper Chromatography
After Paper Chromatography
Distance Alcohol Traveled: ___________ mm Color of Pigment
Distance Traveled (mm)
Rf value
Analysis Questions: 1. Did the leaf you test contain different pigments? Refer to your results to support your answer.
Chromatography of Spinach.08.doc 2. Why did the separation of pigments in the spinach extract occur as it did? (i.e. How does paper chromatography work?)
3. Why are leaves green even though other pigments are present?
4. Based on what you have learned in this lab, explain why leaves tend to change color in autumn in New England.
