Higher Technical School of Agricultural Engineering UPCT
PLANT BIOTECHNOLOGY
Degree/s: Degree in Agro-Food Engineering and Biological Systems Course: 2015/2016
1. Subject information Name Subject area Module Code Degree programme Study plan Centre Type
Plant Biotechnology Specific optional subjects Optional subjects 518109001 Degree in Agro-Food and Biological Systems Engineering 2013 Higher Technical School of Agricultural Engineering (ETSIA) Optional
Teaching period
Four-month period
Language
English and Spanish
ECTS
3
Hours / ECTS
30
Four-month period
2nd
Course
Total workload (hours)
4º
90
2. Teacher information Lecturer in charge Department Knowledge area Office location Telephone email URL / WEB Office hours Location
Teaching and research profile Teaching experience
Research lines
Antonio A. Calderón García Agricultural Science and Technology Plant Physiology 2.17. 2nd Floor, ETSIA 968 325542
Fax
[email protected] http://www.upct.es/~etsia/ Updated in “Aula virtual” at the start of the academic year Office 2.17, 2nd floor ETSIA Degree in Chemical Sciences (Universidad Murcia) PhD in Biological Science (Universidad Murcia) Titular Professor at university (Plant Physiology) At the UPCT from 1999 Teaching quinquennia: 4 Heavy-metal hyper-accumulation and tolerance in plants, molecular and physiological biomarkers of stress in plants, conservation of threatened species by biotechnological tools, production of bioactive compounds by cell cultures Six-year research periods: 3
Work experience Other
Lecturer in charge Department Knowledge area Office location Telephone email URL / WEB Office hours
Teaching innovation
Mª Ángeles Ferrer Ayala Agricultural Science and Technology Plant Physiology Office 2.19, 2nd Floor, ETSIA 968 325535
Fax
[email protected] http://www.upct.es/~etsia/ Updated in “Aula virtual” at the start of the academic year
Location
Office 2.19, 2nd floor ETSIA
Teaching and research profile
Degree in Biological Sciences (Universidad Murcia) PhD in Biological Science (Universidad Murcia) Marie Curie Post-doctoral Fellow ENSAT (Toulouse) Titular Professor at university (Plant Physiology)
Teaching experience
At the UPCT since 2001 Teaching quinquennia: 4 Teaching Excellent Award in 2008/2009 from UPCT
Research lines
Heavy-metal hyper-accumulation and tolerance in plants, molecular and physiological biomarkers of stress in plants, conservation of threatened species by biotechnological tools, production of bioactive compounds by cell cultures Six-year research periods: 3
Work experience Other
Lecturer in charge Department Knowledge area Office location Telephone email URL / WEB Office hours Location
Teaching and research profile
Teaching experience
< 1 year Teaching innovation
Catalina Egea Gilabert Agricultural science and technology Plant Physiology Office 2.18, 2nd floor ETSIA + 34 968 32 55 20
Fax
+ 34 968 325435
[email protected] http://www.upct.es/~dcta/ http://taidaa.upct.es/profesorado_master.php Previous appointment by e-mail Office 2.18, 2nd floor ETSIA and/or by e-mail Degree in Chemistry from the University of Murcia PhD Biochemistry from the University of Murcia Post-doctoral Fellow University of Nottingham (United Kingdom) Associate Professor Assistant Professor: January 2000-Feb. 2002 Associate Professor: February 2002-Present 14 years teaching the subject 4 teaching quinquenniums (last 2009)
Research lines
Plant-pathogen interaction. Mechanisms of biological control of diseases. Induction of stresses for resistant plants with high nutritional quality.
Work experience
Working on issues related to plant pathogen interaction since 1994. Three granted Six-year periods:
Other
3. Contents 3.1 Contents according to the study plan of the subject Unit I: Introduction to Plant Biotechnology Unit II: Plant tissue culture Unit III: Transgenic plants
3.2 Theory program (teaching modules and units) Unit I: Introduction to Plant Biotechnology (C1) Chapter 1. Introduction (C2) Chapter 2. The Plant Biotechnology laboratory
Unit II: Plant tissue culture (C3) Chapter 3. Basic methodology. (C4) Chapter 4. Cell cultures. (C5) Chapter 5. Meristem culture. (C6) Chapter 6. Micropropagation (C7) Chapter 7. Somatic embryogenesis (C8) Chapter 8. In vitro production of haploid plants. (C9) Chapter 9. Techniques for plant germplasm storage and cryopreservation (C10) Chapter 10. In vitro production of secondary metabolites Unit III: Transgenic plants (C11) Chapter 11. Transgenic plants (I): Techniques for plant transformation. (C12) Chapter 12. Transgenic plants (II): Transgenics in crop improvement. (C13) Chapter 13. Transgenic plants (III): Biosafety concerns and regulatory framework.
3.3 Practice program (name and description of every practical) P1. P2. P3. P4. P5. P6.
Plant cell culture initiation and characterization. Isolation of the shoot apical meristem. In vitro propagation and acclimation of plants. Analysis of antioxidant compounds produced by plant cell cultures. Protoplast isolation and fusion. Agrobacterium-mediated transformation of leaf disks.
4. Bibliography and resources 4.1 Basic bibliography For all units: Agricultural Biotechnology (1998). Altman, A. (ed). Marcel Dekker, Inc. Nueva York. Introduction to Plant Biotechnology, 3ª edición (2009). Chawla, H.S. CRC Press Introducción a la biotecnología vegetal métodos y aplicaciones (2001). Caballero, J.L. CajaSur For Unit 2: Functions and Biotechnology of Plant Secondary Metabolites 2nd ed (2010). Wink, M. Wiley-Blackwell Micropropagation.-Technology and Application (1993). Debergh, P.C. y Zimmerman, R.H. (eds). Kluwer Academic Publishers. Dordrecht. Plant Cell Culture Protocols, 2ª edición (2006). Loyola-Vargas, V.M. y Vázquez-Flota, F. (eds). Humana Press. Totowa For Unit 3: Plant Tissue Culture Engineering (2006). Gupta, S.D. y Ibaraki, Y (eds). Springer. Dordrecht. http://unicorn.bib.upct.es/uhtbin/cgisirsi/x/0/0/57/28/3229/X?user_id=WEBSERVER
4.2 Complementary bibliography Functions of Plant Secondary Metabolites and their Exploitation in Biotechnology (1999). Wink, M. (ed). Sheffield Academic Press. Sheffield.
4.3 On-line resources and others “Aula virtual”