Preparation of Bladder Catheters - University of Florida

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SOP Number: CP059.00 Title: Preparation of Bladder Catheters Revision No: Replaces: 00 N/A Author: Kristin Smith I

Date in effect: Page: 7/15/2010 Page 1 of 4 MAL Director: Dr. Peggy Borum

FSHN Chair: Dr. Neil Shay

PURPOSE The purpose of preparation of bladder catheters is to have custom bladder catheters for the survival surgeries.

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SCOPE This procedure provides instructions for the preparation of urinary catheters.

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RESPONSIBILITIES It is the responsibility of Metabolic Assessment Laboratory personnel to follow this procedure. It is the responsibility of supervisory personnel to ensure compliance with this procedure and to train employees and students responsible for performing this procedure. Students will report accidents to the principal investigators immediately.

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REFERENCES N/A

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REAGENTS AND MATERIALS V.A. V.B. V.C. V.D. V.E. V.F.

V.G. V.H. V.I. V.J. V.K. V.L.

Silastic Medical Grade Tubing 0.062 ID by 0.125 OD (Dow Corning Cat. No. 602-285) Silicone Rubber Sealant (Perfecto Manufacturing Aquarium Sealant, for example) Large needle PP Macro Filter, mesh opening 210 microns, thickness 320 microns, (Spectrum Laboratories Inc.,Cat. No. 146428, control number 610473, for example) 94-0 prolene blue monofilament polypropylene suture (Ethicon Cat. No. 8621H, for example) Items needed for the external end of the catheter: V.F.1. 16 Gauge Intramedic Luer Stub Adapter (Clay Adams, Cat. No. 7561, for example) V.F.2. Locking Luer Male Adapter-Short (Abbott, Cat. No. 5396, for example) Small, sharp scissors. Ruler. Fine-pointed forceps. Hemostat. Small, brown paper bag. Kimwipes.

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SOP Number: CP059.00 Title: Preparation of Bladder Catheters Revision No: 00

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EQUIPMENT N/A

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SAFETY PRECAUTIONS VII.A. Members of the MAL have been trained extensively in the procedures described in this SOP. VII.B. Members of the MAL have completed animal contact training in order to work in the PNICU.

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DEFINITIONS VIII.A. Standard Operating Procedure (SOP) – Standard Operating Procedure is a document that provides instructions for completing a specific task in the lab. VIII.B. Metabolic Assessment Laboratory (MAL) – The Metabolic Assessment Laboratory is the laboratory that will use this SOP. VIII.C. Piglet Neonatal Intensive Care Unit (PNICU) – The PNICU is a unit where the piglet is monitored by 24 hour care and routine check-up parameters using PNICU SOPs conducted by the MAL.

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PROCEDURE IX.A. Cut a 2-3 mm Length of Silastic Tubing to use as the Catheter Collar. IX.B. Cut 30cm Length of Silastic Tubing. IX.C. Stretch the collar over the closed hemostat and then place the tip of the hemostat approximately 5 mm into the longer section of tubing. IX.C.1. Use the fine pointed forceps to pull the collar onto the catheter and pull the collar 2-3 cm from the tip of the catheter. IX.D. Cut off the tip of the silastic tubing that may have been damaged during the previous manipulation to a length of 1 cm from the tip. IX.D.1. Clean the surface with 70% ethanol to remove any oily residue from the surface. IX.E. Secure the collar to the silastic tubing with a small amount of silicone rubber sealant. IX.F. Use the large needle to ensure that the sealant is applied thoroughly to the edges of the collar while the catheter tubing is stretched between the thumb and forefinger. IX.G. Use a Kimwipe to ensure that the surface is smooth and that excess silicone rubber has been removed. IX.H. Cut several openings in the silastic tubing between the collar and the tip. IX.I. Allow the sealant to dry and cure for 24 hours. IX.J. Cut a strip of polypropylene mesh 1 to 1.5cm longer than the length of the catheter from the top of the collar to the tip of the catheter and 2 cm wide.

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SOP Number: CP059.00 Title: Preparation of Bladder Catheters Revision No: 00

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Date in effect: 7/15/2010

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IX.K. Wrap the mesh around the catheter extending 3-6mm below the tip and above the collar. Secure the mesh pouch to the catheter using polypropylene sutures beginning at the tip, travelling to the collar, and returning to the tip. IX.L. Wrap the catheter in a brown paper bag and autoclave.

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SOP Number: CP059.00 Title: Preparation of Bladder Catheters Revision No: 00

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ATTACHMENTS X.A. For a list of materials and their locations, refer to SOP# CG098.00.

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