Polysaccharide-based chiral HPLC Phases for Better Performance

Polysaccharide-based chiral HPLC phases for better performance

Kromasil® AmyCoat™ Kromasil® CelluCoat™

Designed to stretch the limits. Kromasil ® AmyCoat ™ and Kromasil ® CelluCoat ™ stretch the limits for analytical and industrial chiral chromatography. The silica is based on an in-house developed matrix and coated with a functionalized amylose or cellulose selector. High resolution, excellent selectivity, no pressure limits* and stable performance when switching between compatible mobile phases are some important benefits.

ANALYTICAL CHROMATOGRAPHY N O P R E S S U R E L IM I T S

H I G H FL O W R A T E S

SMALL PARTICLES (3 μm)

FASTER ANALYSES

H I G H R E S O L U T IO N

U N I Q U E C O A T IN G M E T H O D

BETTER ANALYSES

E X C E L L E N T S E L E CT I V I T Y

BETTER ANALYSES

PREPARATIVE CHROMATOGRAPHY U N I Q U E S IL I C A M A T R I X

M E C H A N I C A L L Y S T R ON G

LONGER LIFETIME

W I D E R A N G E OF P A R T I C L E S

C O N S I S T E NT P E R F O R M A N C E

U N I Q U E C O AT I N G M E T H O D

H I G H E R L OA D A B I L I T Y

* Kromasil AmyCoat and Kromasil CelluCoat can withstand flow rates equivalent to pressures of up to 400 bar—i.e. the approximate limit for most HPLC systems.

EASIER TO SCALE UP

HIGHER PRODUCTIVITY

Peak performance. Expect nothing less. To speed up and simplify method development, we have removed some of the restrictions for coated polysaccharide phases. In analytical scale chromatography, 3 μm particles and the absence of pressure limits allow faster chromatography with better results than ever before.

BETTER RESULTS

FASTER RESULTS

Both Kromasil AmyCoat and Kromasil CelluCoat

Not only can you get better results with Kromasil

show great enantioselectivity for many different

AmyCoat and Kromasil CelluCoat you also get

racemates. In a separate section of the brochure,

them faster. Thanks to a product characteristic

you will find an application guide showing exactly

like the absence of pressure limits, you can run

the performance levels you can expect.

analytical chromatography at very high flow

By having access to 3 μm particles, it enables a higher plate count and resolution for analytical

rates and save time. The high selectivity and 3 μm particles are also

chromatography. Combined with great selectivity,

important factors in speeding up the method. You

this facilitates the separation of the enantiomers.

achieve an improved resolution, which, in turn, allows a higher flow rate.

Kromasil® AmyCoat™ and Kromasil® CelluCoat™ are available in particle sizes down to 3 μm.

Kromasil® AmyCoat™ Selectivity and resolution comparison—Kromasil AmyCoat 3 μm and Chiralpak AD-H (5 μm). Kromasil AmyCoat, 3 μm

␣

Chiralpak AD-H

500

800 0

␣ = 1.5 Rs= 7.3

Rs= 5.8

605 0

000

400 0

0 50

205 0

0 0

5

10

15 [min.]

0

Common conditions Solute: Carbinoxamine Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1) Column size: 4.6 × 150 mm

5

10

.4 4

0 .3 0 .2

.5

␣ = 1.1

.4

Rs= 1.3

Particle size: 3 μm Column length: 150 mm Flow rate: 1 ml/min

0

AmyCoat 3 μm

Chiralpak AD-H*

Ambucetamide

1.4

1.4

4.8

4.2

Carbinoxamine

1.5

1.5

7.3

5.8

Ketoprofen

1.4

1.3

4.6

4.3

Naproxen

1.2

1.2

3.4

3.1

Oxamniquine

1.2

1.2

3.3

3.1

Proglumide

2.7

2.8

11.8

9.0

Sulindac

1.3

1.3

4.8

3.9

(* 5 μm)

Fast analytical chromatography


␣ = 1.1

00

Rs= 2.1

00


= 1.8 ␣ =␣2.3 = 6.9 s Rs=R4.7

.3

00 00

00

00

.1

00

00

0

0

␣ = 1.7 Rs= 3.5

00

Temperature: 25 °C Detection: UV 220 nm

Baseline separation by using 3 μm particles

00

0 0

Common conditions Solute: trans-2-Phenyl-1-cyclohexanol Mobile phase: Heptane/2-Propanol (95/5) Column diameter: 4.6 mm

Particle size: 3 μm Column length: 150 mm Flow rate: 4.5 ml/min

00 ␣␣= =2.21.8 R = 5.6 Rs=s 3.8 00

00

00 .2

0 .1

15 [min.]

Rs Chiralpak AD-H*

Flow rate: 0.5 ml/min Temperature: 22 °C Detection: UV 226 nm

Difference in resolution—Kromasil AmyCoat 3 μm vs. 5 μm. Particle size: 5 μm Column length: 150 mm Flow rate: 1 ml/min

AmyCoat 3 μm

␣ = 1.5

2

4

6 [min.]

Common conditions Solute: Tröger’s base Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1) Column diameter: 4.6 mm

0 0

1

2

3 [min.]

0 0.75 1.5 [min.]


Baseline separation in less than 1 minute

Kromasil® CelluCoat™ Selectivity and resolution comparison—Kromasil CelluCoat 3 μm and Chiralcel OD-H (5μm). Kromasil CelluCoat, 3 μm

500

␣ = 2.3

00 0 50

␣

Chiralcel OD-H

Rs= 13.2

400 0

CelluCoat 3 μm

␣ = 2.0 Rs= 8.3

305 0 200 0

00 0

105

50 0

0 0

2

4

6

8 [min.]

Common conditions Solute: trans-Stilbene oxide Mobile phase: Heptane/2-Propanol (90/10) Column size: 4.6 x 150 mm

0

2

4

6

8 [min.]

Rs Chiralcel OD-H*

CelluCoat 3 μm

Chiralcel OD-H*

Trans-Stilbene oxide

2.3

2.0

13.2

8.3

Benzoin

1.5

1.5

8.6

5.7

TFAE

2.9

2.9

14.7

11

Tröger’s base

1.4

1.4

3.7

2.7

Oxprenolol

5.6

5.5

18.1

15.1

Naproxen

1.2

1.2

2.9

2.2

Proglumide

2.0

2.0

7.6

3.2

* (5 μm)

Flow rate: 1 ml/min Temperature: 25 °C Detection: UV 229 nm

Fast analytical chromatography Particle size: 5 μm Column length: 250 mm Flow rate: 1 ml/min





0 50

␣ = 2.2

00

␣ = 2.3

00

Rs= 9.0

50

Rs= 10.8

␣ = 2.3

00

␣ = 2.2

Rs= 4.7

50

Rs= 3.8

00

00 00 50

0

5

10 [min.]

Common conditions Solute: trans-Stilbene oxide Mobile phase: Heptane/2-Propanol (90/10) Column diameter: 4.6 mm

0

0

5

0

50

0

0

␣ = 2.2 Rs= 3.0

00 00

50

5

0 0

5

10 [min.]

0 0

1

2

3 [min.]

0 0

1 [min.]

0

1 [min.]


Baseline separation in less than 30 seconds

Kromasil® AmyCoat™ and Kromasil® CelluCoat™ —faster results, better results.

Makes everyday work so much easier. Kromasil AmyCoat and Kromasil CelluCoat let you perform method development without interference from restrictive parameters. Forget pressure limits, long equilibration times and degradation in performance when switching from one compatible mobile phase to another—they simply do not exist anymore.

NO PRESSURE LIMITS

SHORT EQUILIBRATION TIMES

User-friendliness, as we see it, is the ability to

Column equilibration is a time-consuming activity

perform method development without being

when running chiral chromatography. Higher

restricted by various parameters. One well-known

flow rates allow Kromasil AmyCoat and Kromasil

restriction for coated polysaccharide phases is the

CelluCoat to show substantially shorter equilibration

general pressure limit over the bed. Kromasil

times than other coated phases on the market.

AmyCoat and Kromasil CelluCoat withstand flow

In general, long equilibration times are most

rates equivalent to pressures up to 400 bar—which

pronounced when switching mobile phases

is about the limit for the HPLC system itself. This

containing basic additives to acidic additives or

makes it possible to run analytical chromatography

the other way around. The test with a Kromasil

a lot faster.

CelluCoat 3 μm column switching between two compatible mobile phases shows how short

STABLE PERFORMANCE

equilibration times you can get.

From a stability perspective, you can run Kromasil

There are also two tests illustrating short

AmyCoat and Kromasil CelluCoat using compatible

equilibration times and additive switch for both

normal, polar organic and reversed mobile phases.

Kromasil AmyCoat and Kromasil CelluCoat with

Switching between compatible normal to polar

absolutely no sign of memory effects.

organic mobile phases will not lead to any degradation in performance, no need for solvent dedicated columns. To show the stability in column efficiency for Kromasil CelluCoat, we have performed mobile phase switches and visualized them after each switch with a Knox plot.

Kromasil® AmyCoat™ Stable performance—No pressure limits—Freedom to switch solvents Identical result

Identical result After

Before

Test sequence

Solute: trans-Stilbene oxide

00 0

␣ = 3.1 Rs= 15.5

00 0 00 0 0 0

5

Mobile phase

Solute: trans-Stilbene oxide

Flow rate [ml/min]

Run time [h]

Total back-pressure [bar]

1. Heptane/2-Propanol (90/10)

4.3

2

345

2. 2-Propanol

0.7

2

260

3. Acetonitrile

1

2

58

4. Ethanol

1

2

188

5. Heptane/2-Propanol (90/10)

1

2

72

00

00

00

0

10 [min.]

Common conditions Stationary phase: Kromasil AmyCoat, 3 μm Column size: 4.6 × 150 mm Temperature: 25 °C

0

Mobile phase: Heptane/2-Propanol (90/10) Flow rate: 1 ml/min Detection: UV 229 nm

Short equilibration times—Freedom to switch additives Identical result

Identical result

Mobile phase 1 Solute: Carbinoxamine Detection: UV 228 nm 00

Mobile phase 2 Solute: Ketoprofen Detection: UV 250 nm 00

␣ = 1.5

Mobile phase 1 Solute: Carbinoxamine Detection: UV 228 nm

␣ = 1.2

50

Rs= 5.0

00

Rs= 3.4

00

␣ = 1.5 Rs= 5.0

00

00

Equilibration Mobile phase 2 50 Duration: 18 min 0

00

0 0

2

4 [min.]

Common conditions Stationary phase: Kromasil AmyCoat, 3 μm Column size: 4.6 x 150 mm

␣ = 3.1 Rs= 15.6

Equilibration 00 Mobile phase 1 Duration: 12 min 0 0

2

4

6

8 [min.]

Mobile phase 1: Heptane/2-Propanol/DEA (90/10/0.1) Mobile phase 2: Heptane/2-Propanol/TFA (90/10/0.1)

0

Flow rate: 2 ml/min Temperature: 25 °C

2

4 [min.]

5

10 [min.]

Kromasil® CelluCoat™ 8

120 selectivity back−pressure

110

Reduced plate height

Change rel. start value [%]

Stable performance—No pressure limits—Freedom to switch solvents

100 90

7 6 5

Start

4

After 100% Acetonitrile (3h)

3

After 100% 2-propanol (3h)

2 1

0

80 0

10

20

30

40

0

50

1

time [h] Conditions Solute: trans-Stilbene oxide Stationary phase: Kromasil CelluCoat, 3 μm Column size: 4.6 x 50 mm

0.1

0.2

0.3

0.4

0.5

Common conditions Solute: trans-Stilbene oxide Stationary phase: Kromasil CelluCoat, 5 μm Column size: 4.6 x 250 mm

Mobile phase: Heptane/2-Propanol (90/10) Flow rate: 7 ml/min Temperature: 25 °C

Identical result

Identical result

Mobile phase 1

Mobile phase 2

50

Mobile phase 1 50

50

= 2.3

00

= 1.4

00

Rs= 10.0

50 00 Equilibration Mobile phase 1 50 Duration: 15 min 0

00 Equilibration Mobile phase 2 50 Duration: 10 min 0

00 50 0 0

2

0

4 [min.]

Common conditions Solute: trans-Stilbene oxide Detection: UV 229 nm Stationary phase: Kromasil CelluCoat, 3 μm

= 2.2 Rs= 9.9

00

Rs= 2.6

50

50

1

2

0

3 [min.]

Column size: 4.6 x 150 mm Mobile phase 1: Heptane/2-Propanol (90/10) Mobile phase 2: Ethanol

2

4 [min.]


Short equilibration times—Freedom to switch additives Identical result

Identical result

Mobile phase 1 Solute: Naproxen Detection: UV 226 nm

Mobile phase 2 Solute: Tröger’s base Detection: UV 220 nm 00

= 1.2

00

Rs= 2.7 00 00

Mobile phase 1 Solute: Naproxen Detection: UV 226 nm

= 1.4 Rs= 3.4

00 00

Equilibration Mobile phase 2 00 Duration: 20 min

00

Equilibration 00 Mobile phase 1 Duration: 20 min 00 00

0 2

4

6

8

10 [min.]

Common conditions Stationary phase: Kromasil CelluCoat, 3 μm Column size: 4.6 x 150 mm

= 1.2 Rs= 2.6

00

00 0

0.6

Reduced velocity

Short equilibration times—Freedom to switch solvents

0

= 2.2 = 2.1 = 2.1

0 0

2

4

6

8 [min.]

Mobile phase 1: Heptane/2-Propanol/TFA (90/10/0.1) Mobile phase 2: Heptane/2-Propanol/DEA (90/10/0.1)

0


2

4

6

8

10 [min.]

Mobile phase: Heptane/2-Propanol (90/10) Flow rates: 0.1-1.2 ml/min Temperature: 25 °C

Works all the way. Kromasil products are well known for their ability to work along the whole spectrum from analytical to industrial scale chromatography. Kromasil AmyCoat and Kromasil CelluCoat are no exception.

SIMPLIFIES METHOD DEVELOPMENT

4000

With particle sizes from 3 μm to 10 μm giving

3500

identical selectivity, Kromasil AmyCoat and Kromasil excellent performance. As for all Kromasil products, you can perform the required method development in analytical scale columns and then scale up to a larger column. You can, for example, use 3 μm particles in an analytical scale column and translate all the generated data to a larger column packed with 10 μm particles. If you plan to scale up the process, we suggest you use an analytical column packed with 10 μm particles right from the start. 0

B a c k -p r e s s u r e i n c r e a s e [ % ]

CelluCoat make it easy to scale up while retaining

Kromasil CelluCoat, 10 μm (7.2 bar*) 3000 Kromasil AmyCoat, 10 μm (9 bar*) 2500 Chiralcel OD, 20 μm (0.8 bar*) 2000 Chiralpak AD, 20 μm (0.8 bar*) 1500 (* initial bed pressure) 1000 500 0 1 (70 bar)

2 (70 bar)

3 (70 bar)

4 (70 bar)

5 6 (100 bar) (100 bar)

Cycles of packing (piston pressure) N.B. The relative back-pressure increase is a measure of the degree of degradation of the material after repeated packings. Actual particle size for Chiralcel and Chiralpak is about 3 times larger than that for Kromasil, which explains the difference in initial back pressure (back-pressure is inversely proportional to the square of the particle size).

The test was designed to exert greater than 0

normal mechanical stress on the chiral stationary

Easy to scale up

phases, and is performed at a packing pressure

0

above the maximum 50 bar recommended by the manufacturer of Chiralcel OD and Chiralpak AD.

0 0

5

10

15 [min.]

3-CelluCoat 5-CelluCoat • Kromasil 10-CelluCoat • ␣Kromasil • ␣Kromasil = 2.4 R = 13.9 = 2.4 R = 11.4 ␣ = 2.4 R = 10.0 s

Common conditions Column size: 4.6 × 150 mm Mobile phase: Heptane/2-Propanol (90/10) Flow rate: 0.5 ml/min

s

s

FULLY BACK-INTEGRATED We manufacture the super wide pore silica for Kromasil polysaccharide products and perform all

Temperature: 25 °C Solute: trans-Stilbene oxide Detection: UV 229 nm

subsequent steps leading to the final product. This means you get a product with full traceability. Every manufacturing step is ensured through our

MECHANICALLY STRONG

detailed quality system, and the final product is

Mechanical strength is an important product

never released until it has passed our rigorous

lifetime parameter. Kromasil AmyCoat and

quality control tests. Having all the manufacturing

Kromasil CelluCoat have a mechanically strong

steps in-house also gives us a strong position in

spherical silica which withstands repeated

terms of production capacity.

cycles of packing.

Kromasil® AmyCoat™ Carbinoxamine Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1) Flow rate: 0.7 ml/min

Preparative applications Analytical injection Sample size: 10 μg Detection: UV 254 nm

Preparative injection Sample size: 7.5 mg Detection: UV 280 nm

00

00

␣ = 1.5

00

00

00 00

00

00 0

0 0

5

10

0

15 [min.]

5

Purity [%] Yield [%]

Warfarin Mobile phase: Heptane/2-Propanol (85/15) Flow rate: 1 ml/min

Equivalent loading on a 50 × 250 mm column:

Analytical injection Sample size: 8.5 μg Detection: UV 230 nm

10

Enantiomer 1 100 99.7

0.9 g

15 [min.]

Enantiomer 2 99.7 100

Preparative injection Sample size: 17.1 mg Detection: UV 325 nm

50 0 00 0 50

00

␣ = 3.3 00

00

00

50 0

0 0

10

20

30 [min.]

0

5


Common conditions Stationary phase: Kromasil AmyCoat 10 μm Column size: 4.6 x 250 mm

10

15

20

25

Enantiomer 1 99.7 99.4

2.0 g

30 35 [min.]


Temperature: 25 °C

EASY TO SCALE UP Conditions: Column size: 4.6 × 150 mm Flow rate: 0.5 ml/min

500

Conditions: DAC system: NovaSep Pack-n-Sep, 50 mm i.d. Bed length: 135 mm Flow rate: 60 ml/min

200 0

␣ = 1.4

00 0 50

00 5 0 80

Rs= 2.6

␣ = 1.3

500

Rs= 2.4

00 0 50

600 0 00 0

0 5

10

15

20 [min.]

Common conditions Stationary phase: Kromasil AmyCoat, 10 μm Mobile phase: Heptane/2-Propanol (90/10) Solute: Trifluoro-anthrylethanol

␣ = 2.9 Rs= 9.9

5

10

Detection: UV 254 nm Temperature: 20 °C

15

20 [min.]

␣ = 3.0 Rs= 7.9

5 20

0 0

000 0 805 0 60 0 0 40

50

0 0

Conditions: DAC system: NovaSep Pack-n-Sep, 50 mm i.d. Bed length: 132 mm Flow rate: 60 ml/min

00 0

40 5 20

50

Conditions: Column size: 4.6 × 150 Flow rate: 0.5 ml/min

0 0

5

10

15

20

25

30 35 [min.]

Common conditions Stationary phase: Kromasil CelluCoat, 10 μm Mobile phase: Heptane/2-Propanol (90/10) Solute: Trifluoro-anthrylethanol

0

5

10

15

Detection: UV 254 nm Temperature: 20 °C

20

25

30 35 [min.]

Kromasil® CelluCoat™ Loadability comparison Kromasil CelluCoat, 10 μm

Chiralcel OD, 10 μm

Analytical injection: 20 μg

Kromasil CelluCoat, 10 μm

Analytical injection: 20 μg

0

60

␣ = 1.2

00

00

40

0

Preparative injection: 5 mg Collected fractions size: 0.5 min

00

␣ = 1.3

0

Chiralcel OD, 10 μm

Preparative injection: 5 mg Collected fractions size: 0.5 min

00

00

00 0

20

00

0

00

00

0

0 0

5

10

15

20

25 [min.]

0 0

5

10

15

20

25 [min.]

0 0

5

10

15

20

25 [min.]

0

5

10

Enantiomer 1

Kromasil CelluCoat Daicel Chiralcel OD

Common conditions Solute: Naproxen Mobile phase: Heptane/2-Propanol/TFA (90/10/0.1) Column size: 4.6 x 250 mm

Purity [%] 91.2 91.4

Purity [%] 94.4 96.6

Binaphtol Mobile phase: Heptane/2-Propanol (90/10)

Preparative applications Preparative injection Sample size: 1 mg Detection: UV 350 nm

␣ = 1.2

00

0

0 00

0

0

0

0 0

5

10

15

20

25 [min.]

5

0 Purity [%] Yield [%]

Metoprolol Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1)

Equivalent loading on a 50 × 250 mm column:

0 0

00

Analytical injection Sample size: 10 μg Detection: UV 223 nm

10

15


20

25 [min.]

0.1 g


Preparative injection Sample size: 20 mg Detection: UV 290 nm

00 0 00

␣ = 2.4

00

00

00 00 00 0

0 0

5

10

15

20 [min.]

0


Common conditions Stationary phase: Kromasil CelluCoat, 10 μm Column size: 4.6 x 250 mm

5

10

Enantiomer 1 100 97.9

Temperature: 25 °C Flow rate: 1 ml/min

15

20 [min.]


20

25 [min.]

Enantiomer 2 Yield [%] 73.3 46.7

Temperature: 25 °C Flow rate: 0.5 ml/min

Analytical injection Sample size: 10 μg Detection: UV 223 nm

15

2.4 g

Yield [%] 50.1 24.9

Taking a closer look. Kromasil® AmyCoat™ CHIRAL SELECTOR

Before testing conditions not mentioned,

The coated selector is tris-(3,5-dimethylphenyl)

please contact the Kromasil application group,

carbamoyl amylose.

[email protected] PRODUCT ASSORTMENT* 3 μm - AmyCoat

5 μm - AmyCoat

10 μm - AmyCoat

4.6 x 50 mm

Kromasil 3-AmyCoat 4.6 x 50 mm


___

4.6 x 150 mm




4.6 x 250 mm




10 x 250 mm

___

Kromasil-5-AmyCoat 10 x 250 mm

Kromasil-10-AmyCoat 10 x 250 mm

21.2 x 250 mm

___



HN O

O HN

O

O *

O

O O

O

O NH

HN

O O O

O

O HN

NH

O O * n

tris-(3,5-dimethylphenyl)carbamoyl amylose

PARTICLE SIZES

* Other column dimensions available upon request.

PRODUCT CODES Kromasil X-AmyCoat followed by column diameter and length. X indicates particle size: 3, 5 or 10 μm. For example: Kromasil 3-AmyCoat 4.6 x 150 mm Kromasil AmyCoat is available in 3 and 5 μm as pre-packed columns, and in 10 μm as pre-packed column and bulk material. COMPATIBLE MOBILE PHASES 0

10

20

30

40

50

60

70

80

90

2-Propanol in Alkane Ethanol in Alkane Methanol in Alkane* 2-Propanol in Acetonitrile Methanol in Ethanol Methanol in Acetonitrile Methanol in CO2 (SFC) *Due to limited miscibility of methanol in alkanes, ethanol should be added as a mediator when exceeding 5% methanol.

Compatible conditions

Further investigations required

100 %

ORDERING INFORMATION Order online at: www.kromasil.com/online [email protected]

Or contact: Eka Chemicals, Separation Products, SE-445 80 Bohus, Sweden

Tel. +46 31 58 73 60 Fax. +31 58 77 27

Kromasil® CelluCoat™ CHIRAL SELECTOR

Before testing conditions not mentioned,

The coated selector is tris-(3,5-dimethylphenyl)

please contact the Kromasil application group,

carbamoyl cellulose.

[email protected] PRODUCT ASSORTMENT* 3 μm - CelluCoat

5 μm - CelluCoat

10 μm - CelluCoat

4.6 x 50 mm

Kromasil 3-CelluCoat 4.6 x 50 mm


___

4.6 x 150 mm




4.6 x 250 mm




10 x 250 mm

___

Kromasil-5-CelluCoat 10 x 250 mm

Kromasil-10-CelluCoat 10 x 250 mm

21.2 x 250 mm

___



HN

O *

NH

O

HN

O

O

O

O

O

O

O

O

O

*

O O NH HN

O

O

n

O

HN

tris-(3,5-dimethylphenyl)carbamoyl cellulose

PARTICLE SIZES

* Other column dimensions available upon request.

PRODUCT CODES Kromasil X-CelluCoat followed by column diameter and length. X indicates particle size: 3, 5 or 10 μm. For example: Kromasil 3-CelluCoat 4.6 x 150 mm Kromasil CelluCoat is available in 3 and 5 μm as pre-packed columns, and in 10 μm as pre-packed column and bulk material. COMPATIBLE MOBILE PHASES 0

10

20

30

40

50

60

70

80

90

2-Propanol in Alkane Ethanol in Alkane Methanol in Alkane* MTBE in Alkane MTBE in Ethanol Methanol in Acetonitrile Methanol in CO2 (SFC) *Due to limited miscibility of methanol in alkanes, ethanol should be added as a mediator when exceeding 5% methanol.

Compatible conditions

Further investigations required

100 %

The moment you adopt our Kromasil High Performance Concept, you join thousands of chromatographers who share a common goal: to achieve better separations when analyzing or isolating pharmaceuticals or other substances. Not only will you benefit from our patented silica technology, but you gain a strong partner with a reliable track record in the field of silica products. For the past 60 years, Eka Chemicals has pioneered new types of silica. Our long experience in the field of silica chemistry is the secret behind the development of Kromasil, and the success of our Separation Products Group. Kromasil is available in bulk, or in high-pressure slurry-packed columns. The development, production and marketing of Kromasil are ISO 9001 certified. Eka Chemicals is a global company with 3,000 people in 30 countries. It is a business unit within Akzo Nobel, one of the world’s largest chemical groups, with more than 67,000 employees in 80 countries.

Eka Chemicals AB Separation Products SE-445 80 Bohus, Sweden Telephone + 46 31 58 73 60 Fax + 46 31 58 77 27

For NAFTA countries: Akzo Nobel/Kromasil 281 Fields Lane, Brewster NY 10509-2676, USA Telephone + 1 845 276 8223

[email protected] www.kromasil.com

Kromasil®

® Eka Chemicals AB 2007 This publication may not be reproduced in any way without the consent of Eka Chemicals AB.

Polysaccharide-based chiral HPLC Phases for Better Performance

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