Download 20 Aug 2001 ... table is shown below. Table I. Progression of microinfected cells through mitosis. Injection. Cell phase at injection. Numb...
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CORRECTION
The Journal of Cell Biology
Topper et al. Vol. 154, No. 4, August 20, 2001. Pages 707–717. In the original publication of this article, Table I contains a typographical error. In the first column under “Injection,” the third item should read “Cdc34 (≥3 mg/ml)” not “Cdc34CA (≥3 mg/ml).” The corrected version of the table is shown below. Table I. Progression of microinfected cells through mitosis Injection Buffer Cdc34CA (3 mg/mL) Cdc34 (3 mg/mL)
Cdc34 (3 mg/mL) denatured
Cell phase at injection Prophase Prometaphase Prophase Prometaphase Prophase Prometaphase Late prometaphase Anaphase Prophase Prometaphase Prophase
Cdc34 (1.5 mg/mL) Cdc34 (3 mg/mL) with 10 m MG132 or -lactone Prophase
Number of cells Number of cells Number of cells Number of cells injected arrested at prometaphase arrested at metaphase that completed mitosis 8 2 11 4 104 24 11 6 6 3 11 15
89 18
5 5 4
7 15
*These cells spent on average 32.5 min at metaphase, which is significantly different from uninjected cells (P 0.05).