Download Introduction. In this test blue copper sulphate solution is used as an indicator. It will turn violet or purple if added to soluble protein...
Download Introduction. In this test blue copper sulphate solution is used as an indicator. It will turn violet or purple if added to soluble protein. This is the Biuret Test which ...
Download Introduction. In this test blue copper sulphate solution is used as an indicator. It will turn violet or purple if added to soluble protein. This is the Biuret Test which ...
Download Introduction. In this test blue copper sulphate solution is used as an indicator. It will turn violet or purple if added to soluble protein. This is the Biuret Test which ...
Download 2013 Today's Dietitian. Protein Content of Foods. Meat, Poultry, Eggs: Food ( Cooked). Serving Size. Calories. Protein (g). Chicken, skinless 3 oz. 141. 28. Steak.
Download Protein Content of Foods. Meat, Poultry, Eggs: Food (Cooked). Serving Size. Calories. Protein (g). Chicken, skinless 3 oz. 141. 28. Steak. 3 oz. 158. 26. Turkey , roasted. 3 oz. 135. 25. Lamb. 3 oz. 172. 23. Pork. 3 oz. 122. 22. Ham. 3 oz
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Download This unit describes three copper-based assays to quantitate total protein: the biuret method. (Basic Protocol 1). a variation of the Lowry method (Hartree-Lowry ...
Download This unit describes three copper-based assays to quantitate total protein: the biuret method. (Basic Protocol 1). a variation of the Lowry method (Hartree-Lowry ...
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Testing Foods for Protein 2 Introduction In this test blue copper sulphate solution is used as an indicator. It will turn violet or purple if added to soluble protein. This is the Biuret Test which can be used for protein which dissolves in water but not for insoluble material. You will Need
Equipment Test tubes Test tube Rack Glass rod Spatula Mechanical grinder or pestle & mortar Dropping pipette Eye protection Materials Food samples Distilled water Copper sulphate solution (1%) Sodium hydroxide solution (10%)
1. If the food to be tested is liquid, go to 2. If the food to be tested is solid, make an extract. Grind, crush or chop a small amount and put into a test tube to a depth of about 2cm. Add a similar amount of distilled water and stir with a glass rod. Allow to stand for a few minutes.
2. Draw up about 1cm3 of the clear liquid into a pipette and transfer it gently to a test tube containing about 2cm3 dilute sodium hydroxide. Add 2cm3 copper sulphate solution.
Extension work If no colour change occurs there may be protein in the food which is insoluble. If it is present and can be dissolved then the Biuret test can be used to detect. 1. To dissolve protein, crush and grind food as before but put it in a small conical flask. Add enough dilute hydrochloric acid to cover it completely. 2. Boil the mixture gently for 10-20 minutes. If necessary add more acid to prevent it boiling dry. The turn off the heat and allow everything to cool. 3. Using this liquid, repeat the Biuret test starting at stage 2. a violet colour indicates protein which has been made soluble by the treatment with acid.
