2 Description: Strategic business analysis of Marks & Spencer, an iconic British retailer brand: PESTEL, SWOT, Porter's Five Forces and Value Chain Analysis
Download This paper indicates the possibility to use near infrared (NIR) spectroscopy as a rapid method to predict quantitatively the content of ... In the past few years, different methods of analysis had been ... Microchemical Journal 83 (2006)
Download This paper indicates the possibility to use near infrared (NIR) spectroscopy as a rapid method to predict quantitatively the content of ... In the past few years, different methods of analysis had been ... Microchemical Journal 83 (2006)
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12 BASICS OF MS-EXCEL 12.1 INTRODUCTION MS-Excel 2000 is a Windows based application package. It is quite useful in entering, editing, analysis and storing of data
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Complete the . TargetX Application. and all of the following items Items to be mailed to the UAB School of Nursing • Request one (1) official transcript from every
Download 10 Dec 2013 ... essence or aromas of plants are due to volatile or essential oils, many of which have been valued since ... GC-MS analysis is still the most widely used method for routine analysis of ... The FT-IR spectrum of the essenti
Download 10 Dec 2013 ... essence or aromas of plants are due to volatile or essential oils, many of which have been valued since ... GC-MS analysis is still the most widely used method for routine analysis of ... The FT-IR spectrum of the essenti
Komputer Akuntasi Dengan Ms. Excel & Ms. Access 2 BAB I APLIKASI AKUNTANSI MENGGUNAKAN MS.EXCEL A. SIKLUS AKUNTANSI Salah satu definisi akuntansi adalah suatu seni
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Implicaciones médico-legales en las Urgencias quirúrgicas por Aparatos. Seminarios de casos clínicos SCC 1 Vasculares / Neuroquirúrgicos 3
Download will contain an aldehyde functional group. This property can be used as a basis for the analysis of reducing sugars. For example Fehling's solution contains.
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Odor • Requirements for odor – Molecule must be volatile – Molecule must be adsorbed onto the olfactory hair (receptor)
Download will contain an aldehyde functional group. This property can be used as a basis for the analysis of reducing sugars. For example Fehling's solution contains.
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El desarrollo de competencias en las clases de ciencias y matemáticas Figura 2 Hemos seleccionado esta escultura porque ofrece a los alumnos una posibilidad de
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Download 17 Ags 2014 ... hati dalam menunjukkan kesalahan orang lain, rendah hati dalam bergaul, dan rajin menolong dan menyelamatkan orang lain. Mereka tidak mencemarkan pikiran mereka sendiri, sehingga cahaya mereka terang bersinar menembus m
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Purdue-UAB Botanicals Center for Age-Related Disease
MS/MS analysis of Polyphenols Jeevan Prasain Ph.D. Pharmacology & Toxicology UAB
Polyphenols
Phenolic acids and derivatives
Flavonoids
Stilbenes
Lignans
OH
HO
CH2 O
OH CH2 O
HO HO
Flavanols Flavonols Isoflavones
OH
Caffeic acid
OH
OH
O
HO
OH HO
O
OH OH
OH
O
OH
Genistein (Isoflavone)
OH
EGC (Flavanol)
Resveratrol (Stilbene)
O
OH HO
O
OH OH
OH
O
Quercetin (Flavonol)
OH
Enterodiol (Lignan)
LC-MS Profile of the methanolic extract of KDS Column: C8 Aquapore; 7µm, 100 x 4.6 mm i.d. Solvent: CH3CN:H2O (10-40%, run time 30 min)
m/z 415 puerarin
100 m/z 341
Relative Intensity (%)
75
m/z 253 DZN
m/z 415 DZ’N
m/z 547
m/z 283 m/z 445
50
m/z 267 Formononetin m/z 445
25
m/z 431 G’N
m/z 431
0 0
4
8
Time (min)
12
16
What is tandem mass spectrometry? The ability to induce fragmentation and perform successive mass spectrometry experiments (MS/MS) on those fragments. In MS/MS mode, product ion, precursor ion and constant neutral loss scans are performed. Multiple reaction monitoring (MRM) is useful technique for quantitation.
How does it work? Tandem in space means having two mass spectrometers in series. It uses two stages of mass analysis, one to pre-select an ion and the Second to analyze fragments induced, for instance, by collision with An inert gas like argon or helium. This dual analysis can be dual in Space, or dual in time. The most commonly used tandem mass spectrometry is the triple quadrupole (QqQ).
MS/MS data interpretation 1. Identification of molecular ions or quasi-molecular ions. 2. Origin of product ions. 3. Stability and relative intensity of ions.
Product ion spectra apigenin vs. genistein 117
Rel. Int. (%)
100
HO
O OH
O-
O
OH
50
Apigenin
107
65
151
269 225
0
O HO
Rel. Int. (%)
57 O-
133 OH
OH
O
Genistein
29 63 91 0
269
O
50
224 159 180 196 240
107
100
150 m/z m/z
200
250
Product ion spectra of daidzin [A] and puerarin [B] in ESI-MS/MS CH2 OH OH
Product ion spectrum of the ion m/z 305 in LC-MS/MS analysis (Rt. 7.10 min) -
13,847
Relative Intensity (%)
100
139
OMe OH HO
O OH OH
50
137
3'-O-methylcatechin 147 221
179 0
150
200
m/z
287 250
300
Product ion spectrum of the ion m/z 305 in LC-MS/MS analysis (Rt. 7.88 min) -
5105
139
100
Relative Intensity (%)
-
OH OMe HO
O OH OH
137
50
4'-O-methylcatechin 147 111 0
100
221
179 150
200
m/z
287 250
300
Proposed structures of product ions obtained from m/z 305 in ESI-MS/MS H+
OH HO
O
H+ OH
OCH3
O
OCH3
OH OH
m/z 221 H+
-H2O
H+ HO
O
OH
H+ O
OH
m/z 193
OH
m/z 305
HO
-CO
OCH3
OCH3
OH
m/z 139 ring A ion
m/z 287
H+
H+ O
HO
OH
m/z 147
O
OH
m/z 111
m/z 137 ring B dignostic of the ring B methylation
LC-MS/MS-MRM spectra for catechin and 3’and 4’-O-methylcatechin in rat urine
Conclusions z
z
z
z
The CID of isoflavones in ESI-MS generates a series of cluster ions due to the subsequent losses of carbonyl/aldehyde or oxygen. The presence of prominent ions at m/z 133 and 135 in MS/MS of genistein and daidzein indicates that they are not originated from the ring A. Isoflavones glycosides (O- and C-glycosides) generate different product ions in ESI-MS/MS due to the neutral losses of 162 and 120, respectively. The major fragmentation of isoflavones with chlorinated ring B in MS/MS starts with elimination of HCl, whereas the loss from product ions was observed with those chlorine in the ring A.
