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LAPORAN HIBAH PENELITIAN STRATEGIS NASIONAL TAHUN ANGGARAN 2009

PERUBAHAN HISTOPATHOLOGI UDANG WINDU YANG TERINFEKSI Vibrio alginolyticus DAN PENGOBATAN DENGAN BIO-AKTIF ALAMI EKSTRAK RUMPUT LAUT,

Halimeda opuntia.

OLEH Dr.Ir.Arief Prajitno , MS Ir.Prapti Sunarmi Ir.Bambang Budi Sasmita, MS

Dibiayai Oleh Dikjen Dikti.Depdiknas, melalui DIPA UB.No.0174.0/02304.2/XV/2009 , tanggal 31 Des 2008 dan Berdasarkan SK.Rektor No.160/SK/2009, tanggal 7 Mei 2009.

FAKULTAS PERIKANAN DAN ILMU KELAUTAN UNIVERSITAS BRAWIJAYA MALANG

DAFTAR PUSTAKA Anonimous, 1995. Statistik Perikanan Indonesia. Direktur Jendral Perikanan, Jakarta. Anonimous, 1998. Budidaya rumput laut. Direktorat Jendral Perikanan-IDRC. INFIS Manual Seri 62, 1998. Anderson, L.G.,M..Samsudin, M.Shariff and G.Nash,1988 Bacterial septicaemia in juvenile tiger shrimp, Penaeus monodon culture in Malaysian brackish water ponds.Asian Fisheries Science, 2:93-108. Andrews and Pardo,2001.Genetic Manipulation Of Vibrio parahaemolyticus: Three methods.URJ-NCSU.Undergraduate Journal Of N.C.State.Volume I.English. Angka dan MT.Suhartono, 2000. Bioteknologi Hasil Laut. Pusat Kajian Sumberdaya Pesisir dan Lautan IPB.Bogor. Cetakan Pertama. Aoki,,T, T.Kitao,T. Habashi, Y.Wada and M.Sakai, 1981. Proteins and Lipopolysakarides in the membran of Vibrio anguilarum.Dev : Biol.Standart 49.Serodiagnostic and vaccines : 225-232. _____, T.Kanasawa and T. Kitao, 1985. Epidemilogical of drug resistenst Vibrio anguilarum strains.J.of Pathology, 20(2/3):199-208 Baticados, M.CL., C.R.Lavilla-Pitogo, E.R. Cruz Lacierda,L.D. de la Pena and N.A. Sunaz, 1991.Studies on the chemical control of luminescent bacteria, Vibrio harveyi and V.splendidus isolated from diseased Penaeus monodon larvae and rearing water.Dis.Aquat.Org., 9 : 133-139. Bauman, P and L.Bauman, 1977. Biology of The marine Enterobacteria : General Benecca and Photobacterium. Annu.Rev.Microbial, 31 p _____, A.L. Firniss and J.V.. Lee, 1984. Facultatively Anaerobic Gram-Negative Rods.p. 518-545.In : Krieg, N,E.Berge’y Manual of Systematic Bacteriology, Vol 1. Williams &Baltimore, USA Blake, A.A.R.E.,Weaver and D.G.Hollis, 1980.Diseases of humans (other than cholera)caused by Vibrios, Rev.Microbiaol, 34: 341-367. Boer, D. R. dan Zafran. 1992. Karakteristik Beberapa Isolat Bakteri Bercahaya yang Diisolasi dari Udang Windu Penaeus monodon. Jurnal Penelitian Budidaya Pantai, Vol. 8 No. 3. Subbalitkandita Gondol Bali. Hal 93-98. Buchanan, R.E and N.E.Gibbon, 1974 Bergey’s Manual of Determinative Bacteriology, Eight Edition, The Williams and Wilkins Company, Baltimore. Bullock, 1971. Bacterial Diseases of Fish, Book 2A. In Snieskzko and Axelord (Eds) Disease of Fish .T.F.H.Publication.England, 145 p.

Chen, K.M.L. Woo, L.Y.Lenn,and G.L.French, 1989.Vibrio Parahaemolyticus and other halophlic vibrios associated with seafood in Hongkong. J.Appl.Bacteriol, 66: 57-64. Chen, S.c., S.L., Huang and G.H.Kou, 1992. Studies on the epizootiology and pathogenicity of bacterial infections in cultured giant tiger prawn, Penaeus monodon.in Taiwan. In, Disease of cultured penaeid shrimp in Asia and the United State, pp.1950205.Oceanic Institute, Honolulu. Chong, Y.C. and H. Loh, 1984. Hepatopancreas chlamydial and parvoviral infections of farmed marine prawns in Singapore.Singapore Veterinary,

9 : 51-56. Colorni,A,I.Paperna, and H.Gordin, 1981. Bacterial infection in Giilt-helt seabream, Sparus auratus cultured in Elat. Aquaculture, 23 : 257-267. Crosa, J.H., M.H.Schiewe, and S.Falkow, 1977. Evidence for plasmid contribution to the virulence of fish pathogen Vibrio anguillarum. J.Infect.Immunol, 18

(3) :509-513. ______, J.H., L.L.Hodges and M.H. Schiewe, 1980. Curing of plasmid is coorelated with an attenuation of virulence in the marine fish pathogen Vibrio anguillarum. J.Infect.Immunol, 27 (3) :897-902. ______, J.H. and L.L. Hodges, 1981.Outer membrane proteins induced under conditions of biron limitation in the marine fish pathogen Vibrio Anguillarum.J.Infect.Immunol., 31(1) :223-227. Demsey,A.C.C.L.Kitting andR.A.Rosson, 1989. Bacterial variability among individual Penaeid Schrimp digestive tractc. Crustacean , 56 :267-278. Egidius,E.C. andK.Anderssen, 1978. Host specific pathogenicity of strains of Vibrio angguillarum isolated from rainbow trout Salmon gairdneri and saithe Pollachius virens.J.of Fish Diseases, 1 : 45-50. Egusa, S.Y., Takahashi,T.Itami and K.Momoyama, 1988.Histopathology of vibriosis in the kuruma prawn, Penaeus javonicus .Bact.Fish.Pathol.23 :

59-65. Flegel,T.W.,D.F.Fegan,S.Kongsom,S.Vuthimudomkit,S.Sriurairatana,S. Boonyaratpalin, C.Chantanachookhin, J.E.Vickers and Macdonald, 1992).Occurence, Diagnosis and Treatment of Shrimp Diseases in Thailand. Proceeding of a workshop in Honolulu

Hawaii, April 27-30, Gould, R.W., 1977.Development of a new vaccine delivery system for immunizing fish and investigation of the protective antigens in Viberio anguillarum, Ph.D.Thesis.Oregon State University, Corvallis,p.145. Harell, L.W.,H.M. Etlinger, and H.O.Hodgins, 1976. Humoral factors important in resistance of salnid fish to bacterial disease. II.Anti-Vibrio anguillarum activity in mucus and observation on complement.Aquaculture,7 :363370.

Hariati A.M, D.G.R. Wiadnya dan A. Prajitno, 1997..Status of Monodon Baculo Virus (MBV) Infection of Penaeus monodon Broodstock Capture in Madura Strait (Jurnal Penelitian Ilmu-ilmu Teknik Vol. 9. Lembaga Penelitian Universitas Brawijaya. Hastein, T, and J.E.Smith, 1977. A.Study of Vibrio anguillarum from farmed and wild fish using principal components analysis.J.of Fish Biology. 11: 69-75. Horne, M.T., 1982 The pathogenicity of Vibrio anguillarum.In Microbio diseases of fish (Ed.R.J.Robberts).pp 1971-1982.Academic Press.London. Jie,K.Y.Jun,L.Ping,Z.Yan,L.Xingming, X.Huishu and Y.Conghai, 1996. Rapid identification of shrimp bacterial pathogens by enzymatic amplification and digestion of 16 S, RNA gene.World Aquaculture”96. The Annual Meeting of the Word Aquaculture Soc, Bangkok, Jan, 29-Feb 2, 1996. P115. Kamiso, H.N. 1985. Differences in pathogenicity and pathology of Vibrio anguillarum and Vibrio harveyii in chum salmon (Oncorhynchus keta) and English sole (Parophrys vetulus) under laboratory conditions.Ph.D.Thesis submitted to Origon State University, p116. ______, H.N., Triyanto dan Sri Hartati, 1992. Penanggulanagn penyakit Motil Aeromonas Septicaemia (MAS) pada ikan lele (Clarias spp).ARM.Project, Balitbang Pertanian, Deptan, Jakarta, 38 hal. ______, H.N. 1996. Vibriosis pada ikan dan alternatif cara penanggulangannya.J.Perikanan UGM (GMU J.Fish.Sci) 1(1) :78-86. ______, H.N. 1997. Vaccination with Vibrio anguillarum and Vibrio ordalli bacterins againt vibriosis.J.Perikanan UGM.(GMU J.Fish Sci) : 1-8. Kaul, P.N.,1979. The sea’s biomedical potensial.,Impact of science in Society.Vol 29,(2) 123-134. Kitao, T,.T.Aoki, M.,Fukudome, K.Kawano, Y.Wada and Y,Mizuno, 1983. Serotyping of Vibrio anguillarum isolated from diseased freshwater fish in Japan.J.of Fish Disease. 6 : 175-181. Lavilla-Pitogo, C.R., M.C.L.Baticados, , E.R. Cruz Lacierda,L.D. de la Pena.1990. Occurence of luminous bacterial disease of Penaeus monodon larvae in the Philippines.Aquaculture, 91 : 1-13. Lavilla-Pitogo, C.R., L.J., Albright, M.G.,Paner and N.A.Sunaz, 1992. Studies on the sources of luminescent Vibrio harveyi in Penaeus monodon hatcheries. In :M.Shariff, R.P.,Subasinghe and J.R.Arthur (Eds).Diseases in Asian

Aquaculture 1.Fish Health Section, Asian Fisheries Society, Manila, Philippines,pp.157-164. Lay, B. W. 1994. Analisis Mikroba Di Laboratorium. PT Raja Grafindo Persada. Jakarta. 168 hal.

Lihgtner, D.V.T.A. Bell, R.M.Redman, L.L. Mohney, J.M.Natiyidad, A.Rukyani and A.Purnomo, 1992.A Review of some major disease of economic significance in penaeid prawns/shrimps in the Americas and Indopasific. . In :M.Shariff, R.P.,Subasinghe and J.R.Arthur (Eds).Diseases in Asian

Aquaculture 1.Fish Health Section, Asian Fisheries Society, Manila, Philippines,pp.57-80 Lightner, D.V., 1996. A hanbook of shrimp pathology and diagnostic procedurees for diseases of culture penaeid shrimp.The word Aquaculture Society, Baton Rouge, Lousiana, USA. Liston, J. and J.Barros, 1973 Distribution of V.Parahaemolyticus in the natural environment, J.Milk Food Technology, 36 : 113-117 Lightner, D.V., T.A. Bell, R.M. Redman, L.L. Mohley, J.M. Natividad, A. Rukyani, and A. Poernomo. 1992. A review of some major diseases of economic significance in penaeid prawns/srhimp of the Americas and Indopacific. P:57:80. In Shariff, M., R.P. Subasinghe, and J.R. Arthur (Eds), Diseases in Asian Aquaculture I. Fish Health Section, Asian Fisheries Society, Manila, Philippines. Liu, C.I. 1989. Shrimp diseases, prevention, and treatment. P:64-74. In Akiyama, D.M.(Editor), Proceedings of the Southeast Asian Shrimp Farm Management Workshop. Replubic of Singapore. Mangampa, M., S. Tahe, Rachmansyah, dan M. Tjaronge. 1998. Pengaruh kedalaman penempatan tiram, Crassostrea sp dalam tandon sebagai biofilter tambak udang intensif. Laporan hasil penelitian. Balai Penelitian Perikanan Pantai, Maros. Partasasmita, S. dan Zafran. 1989. Invevtarisasi parasit dan penyakit udang windu (Penaeus monodon) di tempat-tempat pembenihan dan penampungan benur di Sulawesi Selatan. Majalah Parasitology Indonesia 3(Edisi Khusus):63-68. Plumb, J.A. 1992. Disease control in aquaculture. P:3-17. In Shariff, M., R.P. Subasinghe, and J.R> Arthur (Eds), Diseases in Asian Aquaculture I. Fish Health Section, Asian Fisheries Society, Manila, Philippines. Suryanto, M. Atmomarsono, dan A. Sudradjat. 1996. Penggunaan tiga jenis kerang sebagai biofilter pada pemeliharaan udang windu (Penaeus monodon) dalam skala laboratorium. Jurnal Penelitian Perikanan Indonesia, II (1):31-38. Tonguthai, K. And P. Chanratchakool. 1992. The use of therapeutic agents in aquaculture in Thailand. p:555-565. In Shariff, M., R.P. Subasinghe, and J.R. Arthur (Eds), Diseases in Asian Aquaculture I. Fish Health Section, Asian Fisheries Society, Manila, Philippines. Long, S, M.S. Mothibeli, F.T.Robb and D.R. Woodd,1981. Regulation of extracelluler alkaline protease activity by histidine in a collagenolytic Vibrio alginolitycus. Strain. J.Gen Microbiol., 127: 193-199.

Lupiani, B, A.M.Baya,B.Magarinos, J.L. Romalde, BS.Roberson, FM.Hetick,A.E.Toranso, 1993. Vibrio minicus and V,cholerae non-isolated from wild and hatchery reared fish.J.of.Fish Pathology, 28 (1) : 15-26. Maftuch, 2006.Outer membrane protein (Omp) Vibrio alginolyticus sebagai vaksin untuk mengendal;ikan penyakit vibriosis yang disebabkan V.alginolyticus pada ikan kerapu tikus di perairan. Ringkasan Disertasi.Program Doktor.Program Studi Ilmu Kedokteran.Universitas Brawijaya Program Pasaca Sarjana Malang. 68 hal. Murdjani, M., 2002.Identifikasi dan patologi bakteri Vibrio alginolyticus pada ikan kerapu tikus (Cromileptes altivelis).Disertasi .Program Pasaca Sarjana Univerisitas Brawijaya Malang,117 hal. Neilands, J.B., 1981. Iron absorption and transport in microorganisms, Ann.

Rev.Of.Nutr., 1:27-46. Noviana, L. 2004. Identifikasi Senyawa Flavonoid dari Propolis Lebah Madu (Apis mellifera) dan Uji Aktivitasnya sebagai Antibakteri Staphylococcus aureus. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Brawijaya. Malang. 61 hal. Pacha, R.E.and H. Wakabayashi, 1985. Characterization and relattedness of Marine Vibrios pathogenenic to fish:Physiology, serology and epidemilogy.J.Bact.100(3) : 295-302. Prajitno, A. E. Y. Herawati dan A. M. Hariati. 1998. Stategi Pengaturan Salinitas dan Pemberian Serbuk Jamur Merang Sebagai Penanggulangan Penyakit Kunang-kunang (Vibrio spp.) Pada Udang Windu di Mini Backyard. Fakultas Perikanan. Universitas Brawijaya Malang. 40 hal. DP4M No. 094/P21PT/DPPM/LITMUD/V/1997. Prajitno,A,1994. Laporan survey Bakteri Vibriosis di Tambak Pantai Utara Jawa. Fakultas Perikanan, Universitas Brawijaya., 50 hal. Prajitno, A. 2004. Patogenitas bakteri Vibrio spp, ditambak udang windu (Penaeus monodon Fab). Diktat Kuliah Parasit dan Penyakit Ikan.Fakultas Perikanan, Universitas Brawijaya Malang, 45 hal.. Prajitno, A. 2005.Diktat Kuliah Parasit dan Penyakit Ikan. Jurusan MSP Fakultas Perikanan, Universitas Brawijaya Malang, 105 hal.. Prajitno, A. 2006. Laporan penelitian pendahuluan uji sensitivitas H.opuntia terhadap bakteri V.harveyi dan V.parahaemolyticus secara invitro.,45 hal.

Pelczar, M. J. dan E. C. S. Chan. 1986. Dasar-dasar Mikrobiologi 2. Alih Bahasa Oleh: Hadi Oetomo et al. UI Press. Jakarta. 997 hal. Plumb, J.A. 1992. Disease control in aquaculture. P:3-17. In Shariff, M., R.P. Subasinghe, and J.R. Arthur (Eds), Diseases in Asian Aquaculture I. Fish Health Section, Asian Fisheries Society, Manila, Philippines. Ransom, D.P.1978. Bacterilogic, immunologic and pathologic studies of Vibrio

spp.pathogenic to salmonids. Ph.D.Thesis. Oregon State University, Carvallis, p.123. Reftie, T., 1982. Preliminary result :Differences between rainbow trout families in resistence againt vibriosis and stess. Dev.Comp.Immunol.Sup.2: 205209. Richards, RH. 1980. Observbations on vibriosis in cultured flatfish. In Fish Diseases (Ed.W. Ahne) pp.75-81.Third COPRAQ-Session, Spriner-Verl;ag, Berlin Rosemark, R.1988. Vibriosis of lobters. In Diseases diagnosist and control in North American Marine Aquaculture.(Eds.C.J.Sinderman and D.V.Lightner).pp. 240-243 .Elseveier, Amsterdam Rukyani, Taufik and Tauchid,1992. Luminescent Vibriosis at Hatchery and preventive. Proceeding of a workshop in Honolulu, Hawaii, April

27-30, Rukyani, A. 1999. Beberapa jenis penyakit pathogen sebagai kendala utam budidaya udang windu dan cara penanggulangannya. Seminar penataan Tambak Berwawasan Lingkungan, 1-4 Nov, Ditjen Perikanan 12 p. Schmidt, U.H.Chmel and C.Cobbs, 1979. Vibrio alginolyticus infection in humans. J.Clin.Microbiology, 10 : 666-668. Seng, L.T. 1994. Parastes and diseases of cultured marine finfish in South East Asia.Pusat Pengkajian Sains Kajihayat, Universitas Sains Malaysia. P25 Sinderman C.J. 1988. Vibriosis in larval oyster. In Disease diagnosis and control in North American marine Aquaculture (Eds.C.J. Sindermann and D.V.Lightner) pp.275-276.Elsevier, New York. Shariff ,M. And Subasinghe.R.P.Subasinghe, (1992) Major diseases of cultured shrimp in Asia : An overview. Proceedings of a workshop in Honolulu,

Hawaii, April 27-28,1992. Susanto, 2000. Halimeda opuntia . Jur.Kelautan, Fakultas Perikanan dan Ilmu Kelautan UNDIP.. 15 hal. Tonguthai, K. And P. Chanratchakool. 1992. The use of therapeutic agents in aquaculture in Thailand. p:555-565. In Shariff, M., R.P. Subasinghe, and J.R. Arthur (Eds), Diseases in Asian Aquaculture I. Fish Health Section, Asian Fisheries Society, Manila, Philippines.

Volk, W.A. and M.F. Wheeler. 1988. Mikrobiologi Dasar. Alih Bahasa : Markham. Edisi 5. Penerbit Erlangga. Jakarta. 396 hal. Winarno, 1996. Teknologi Pengolahan Rumput Laut. Pustaka Sinar Harapan Jakarta., 150 hal Wedemeyer, G., 1970. The role of stress in the disease resistence of fish. In : A symposium on disease of fish and shellfish (Ed.S.F..Snieszko).pp 3035.Spec.Pub.,5.Am.Fish Soc., Washington, DC. Wolke, R.E.1975. Pathology of bacterial and fungal diseases affecting fish. In : Pathology of fishes. (Eds.WE.Ribelin and G.Migaki ) pp.33-116, Univ.of Wisconsin Press, Madison, Wisconsin. Wood, J.W.1992. Diseases of pasific salmon thein prevention and treatment, Second ed. State of Washington, Dept of Fish.Olympia, p.81. Wren, B.W,.1992. Bacterial enterotoxin interactions. In : Molecular biology of bacterial infections, curent status and future prospectives .(Eds.C.E.Hormaeeche , C.W.Penn and C.I.Smith).pp.127-128.Cambridge University.Press. Wu, J.H.Lin,L.Jan, Y.Hsu and L.Chang, 1981. Biological control of fish bacterial pathogen, Aeromonas hydrophyla by bacteriophage. AH.1.J.of. Fish Pathology, 15 (3/4):271-276. Yong, W.S. and L.T.Seng, 1990. A comparative study of Vibrio infections in healthy and diseased marine finfish culture in floating cages near Penang, Malaysia, Asian Fisheries Science, 3 (3) : 353-360. Yoshie.Y,W.wang, Y.P.Shieh and T.Suzuki, 2001.Comcositional difference of phenolic compounds between two seaweeds, Halimeda spp.Department of Food Science and Technology, Tokyo University of Fisheries,5-7, Konan 4-Chome, Minato-ku,Tokyo 108-8477, Japan