paper and some are more attracted to the alcohol or water. In this experiment you learn how to prepare chromatography paper and solution. You will als...
applications of chromatography – identification of an unknown ink sample and the separation ... Calculating Rf values and interpreting the results. In order to obtain a quantitative measure of the extent of movement of a component in a paper chromato
In paper chromatography, the sample mixture is applied to a piece of filter paper, the edge of the paper is immersed in a ... a component in a paper chromatography experiment, we can calculate an "Rf value" for each separated ... All of the solutions
Paper Chromatography Lab Questions. Answer each of the following questions. Base your answers (conclusions) on reasonable interpretations of your data and provide justification. 1. Why is it important to keep the spots applied to the chromatography p
Paper Chromatography Lab Chromatography is a method for analyzing complex mixtures by separating them into the chemicals from which they are made
Paper Chromatography Lab ... 4th – Have your answers checked by the teacher. Keep trying until you are able to identify all 6 pens! Pen A matches # _____
Fisher in rolls of 100 yards (300 feet). Since students will be working in groups of 3 or 4, you will need to cut 30-32 strips of chromatography paper, one for each student. 2. Obtain brands of ink that will yield good results. Pens that work well in
Plant Traveling Lab. TTU/HHMI at CISER. 2010 2 The bands derived in paper chromatography contain the pigments found in the plant. The bands can be cut apart, and
chosen for this experiment because when the eluting solution reaches that line, any inks that are mixtures should be clearly separated. When the solvent front reaches the finish line, the paper should be removed immediately from contact with the mobi
In a previous experiment you have seen how differences in the polarity of molecules can ... The point of this experiment is not to develop a wash-day solution to all ... In paper chromatography substances are applied to a piece of absorbent paper. A
Jan 31, 1996 ... measures the absorption of light by a solution at any wavelength(s) selected by the experimenter. Light visible ... To separate pigments from leaves of a green plant using paper chromatography and to determine .... 180 mL petroleum e
Plants contain chlorophyll, a green pigment, as well as carotenoids, pigments that range in color from red to orange to yellow. Question: Do green leaves also contain other pigments? Hypothesis: Record on the Analysis Sheet. Materials: Isopropyl alco
Instructor guide and answer key. Materials: ... others. If this lab is done in the winter when leaves are not available, spinach leaves can be used. ... 1. Experiment with different types of leaves. Which leaf has the greatest amount of pigment? 2. P
and/or tape your chromatogram into the lab book. • Data table: Determine the Rf values for each component in each ink. Calculations and Data Analysis: 1. Calculate Rf values for each ink or dye using the formula. Rf. = distance traveled by the compou
Chromatography is a method for analyzing mixtures by separating them into the chemicals from which they are made. It can be used to separate mixtures like ink, blood, gasoline, and lipstick. In ink chromatography, you are separating the colored pigme
Lab Activity H5 Paper Chromatography of M&M’s OUTCOMES After completing this lab activity, ... Considering your answers above, how many dyes,
The purpose of this experiment is to use liquid chromatography to separate the component ... 2 Procedure There are two parts to this lab. In part one,
High-Performance Liquid Chromatography High Performance Liquid Chromatography (HPLC) was developed in the late 1960s and early 1970s. Today it is widely applied for
Questions and Conclusions. 1. What criteria were used to identify the ion(s) found in your unknown? Explain your answer in at least three sentences. Include any difficulties in identifying any ions. 2. If you let the experiment run for only 30 minute
SCIENCE EXPERIMENTS ON FILE ™ Revised Edition. 5.31-1. Using Paper Chromatography to Separate Ink. Virgil A. Sestini. Topic. Paper chromatography .... each experiment. If you are working in a lab or in the field, do not work alone. This book assumes
Ink Chromatography Experiment. Safety and First Aid. This section will only deal with the specific safety issues regarding the chemicals involved and used in the video; other safety data may be required depending on the chemicals used. There are like
Download [42] Lensmeyer GL, Onsager C, Carlson IH and Wiebe DA. (1995) Journal of Chromatography A 691: 239}246. [43] LeBelle M, Pike RK, Graham SJ, Ormsby ED and. Bogard H (1996) Journal of Pharmaceutical and Bio- medical Analysis 14: 793}800.
Spinach Chromatography Lab ... Chromatography is a technique for separating and identifying substances in a ... Answers should be in complete
Download PO-CON1575E. On-line supercritical fluid extraction/ supercritical fluid chromatography: A novel approach to the cleaning validation for pharmaceutical manufacturing. HPLC 2015 PSA-SFC-14. Daisuke Nakayama, Hidetoshi Terada, Minori Nak
Paper Chromatography Summary Paper Chromatography is a method used to separate mixtures into their different parts. Paper chromatography has been most commonly used to separate pigments, dyes and inks. To do paper chromatography you need paper with a lot of cellulose fiber (fiber found in wood) and chromatography solution, which is usually made from a mixture of water and alcohol. Paper chromatography works because inks usually contain several different colors. Ink manufacturers mix different amounts of primary colors like blue, red and yellow to make many other colors. During paper chromatography, we can separate basic colored inks that contain different ingredients because some inks are more attracted to the paper and some are more attracted to the alcohol or water. In this experiment you learn how to prepare chromatography paper and solution. You will also use these skills to separate your own samples of inks into their basic colors. In this activity we will: 1. Learn how to do paper chromatography 2. Observe the separation of different types of ink using paper chromatography Materials • • • • • • • • • • •
Large 4-inch cone coffee filter Pencil Ruler Rubbing alcohol Water-based markers Different brands or colors of pens Water Small plate or bowl with flat bottom Medium-sized plastic container Measuring cups Stapler
Preparation 1. Cut the seam of the large 4-inch cone coffee filter so that it opens up into a fan shape. Cut out a rectangular piece that is 8 x 15 cm in size.
2. Position the filter paper so that it is longer going across (see picture below). 3. Draw a line using a pencil across the filter paper that is 1 cm from the top of the sheet and 1 cm from the bottom of the sheet. On the bottom line mark small lines every 2 cm and number each small line. Do not use pen for this step! You have now prepared your chromatography paper.
4. In your plastic container, mix 1/2 cup of rubbing alcohol with 1/4 cup of water. Pour carefully into the shallow plate or bowl. The height of the liquid should be no more that 1 cm. This will be your chromatography solution. Wash and dry the container for the next part of the activity.
Pre-Activity 1. Look at the pens and markers you want to use in this activity. Before beginning the activity, think about the following questions a. What colors are the pens and markers? b. How do you think the manufacturers make these different colors? Do they have to use more than one color of ink? c. Do you think each pen or marker will give the same separation results on the chromatography paper? Why or why not? Activity 1. On each small line of your chromatography paper, make tiny dots with your markers and your pens. Mark one pen or marker on each line. Make a key of which pen or marker goes with each number on a separate piece of paper.
2. Staple the ends of your paper so that they do not touch each other.
3. Carefully place the paper onto the plate or bowl so that the marker/pen dots are just above the chromatography solution. Place a dry plastic container over the paper. Make sure that the paper does not touch the sides of the container.
4. Allow the paper to sit in the solution until the liquid reaches the top line. Do not move or shake the set-up during this time. This may take about 1/2 an hour. Remove the paper and allow it to dry on a paper towel. 5. Observe the results. What do you see? What happened to the different markers?
Extension Activity
Mystery Ink Analysis This experiment is based on techniques used in crime labs to investigate ink samples found at crime scenes. You will need to learn basic chromatography techniques from the activity above before you can try this activity. 1. Gather 4 – 5 different brands of black or blue pens. Have a friend choose one of the pens and write you a message while you are not looking. 2. Now you can use chromatography to guess which pen your friend used. Carefully cut out a piece of their message and dissolve the ink off of the paper using ½ a teaspoon of rubbing alcohol. 3. While the ink is dissolving, prepare a piece of chromatography paper like you did in the paper chromatography activity. 4. You will also need to prepare some chromatography solution or you can re-use solution from a previous experiment. 5. Take your sample pens and make small marks on the lines of the chromatography paper. Make a key of which pen goes with each number on a separate piece of paper. On the last line, make a mark of the dissolved ink from your friend’s message. 6. Place your chromatography paper into the solution and allow the solution to reach the top line. 7. Compare all of your samples to your friend’s sample. Can you guess which pen your friend used to write his or her note? (Hint: Try to match the separated dots (height and color) in the column from your friend’s sample to the pen samples.) Wrap Up Paper chromatography is just one of the methods that scientists can use to separate different mixtures. However, it is the only one that depends on the interactions between paper and ink to make it work. Since its discovery, paper chromatography has been used for many things. For instance, it has been used to identify the components of different inks, to separate plant pigments and it has even been used to help solve crimes! Resources www.teachersfirst.com/lessons/forensics/ink-lab.html www.reachoutmichigan.org/funexperiments/quick/csustan/mrsketch.htm
