Mutations Practice Name Per. _____ There ... Explain your answer. ... Mutations will be passed on to future generations only if they occur in which ty...
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Mutations Practice
Name
Per. ___________
There are several types of genetic mutations: DELETION (a base is lost) INSERTION (an extra base is inserted) Deletion and insertion may cause what’s called a FRAMESHIFT, meaning the reading “frame” changes, changing the amino acid sequence. SUBSTITUTION (one base is substituted for another) Substitutions cause POINT MUTATIONS. If a substitution changes the amino acid, it’s called a MISSENSE point mutation. If a substitution does not change the amino acid, it’s called a SILENT point mutation. If a substitution changes the amino acid to a “stop,” it’s called a NONSENSE point mutation. Complete the boxes below. Classify each as either Frameshift or Point mutations, then specify further with Deletion, Insertion, or Substitution. Use the chart on the following page in order to determine the amino acid sequence. Remember, RNA has Uracil instead of Thymine. . Original DNA Sequence: T A C A C C T T G G C G A C G A C T mRNA Sequence:
AUG UGG
Amino Acid Sequence:
Met - Trp - Asn - Arg - Cys - STOP
Mutated DNA Sequence #1:
T A C A T C T T G G C G A C G A C T
What’s the mRNA sequence? (Circle the change) What will be the amino acid sequence?
AAC
CGC
Met - STOP
YES!
What kind of mutation is this?
Point Substitution - Nonsense T A C G A C C T T G G C G A C G A C T
What’s the mRNA sequence? (Circle the change) What will be the amino acid sequence?
AUG CUG GAA
YES!
What kind of mutation is this?
Frameshift Insertion T A C A C C T T A G C G A C G A C T
What’s the mRNA sequence? (Circle the change) What will be the amino acid sequence?
AUG UGG AAU CGC UGC UGA Met - Trp - Asn - Arg - Cys - STOP
Will there likely be effects?
No!
What kind of mutation is this?
Point Substitution - Silent
Mutated DNA Sequence #4:
T A C A C C T T G G C G A C T A C T
What’s the mRNA sequence? (Circle the change) What will be the amino acid sequence?
AUG UGG AAC CGC UGA UGA
Met - Trp - Asn - Arg - STOP
Will there likely be effects?
Yes!
What kind of mutation is this?
Point Substitution - Nonsense
Mutated DNA Sequence #5:
T A C A C C T T G G G A C G A C T
What will be the corresponding mRNA sequence? What will be the amino acid sequence? Will there likely be effects? What kind of mutation is this?
CCG CUG CUG A
Met - Leu - Glu - Pro - Leu - Leu
Will there likely be effects?
Mutated DNA Sequence #3:
UGA
AUG UAG AAC CGC UGC UGA
Will there likely be effects?
Mutated DNA Sequence #2:
UGC
AUG UGG AAC CCU GCU GA Met - Trp - Asn - Pro - Ala
Yes! Frameshift Deletion
Analysis Questions: 1.
Which type of mutation (insertion, deletion, or substitution) appears to have the most significant impact on an amino acid sequence? Explain your answer. Insertions and Deletions – they cause frameshifts!
2.
Most mutations are “silent.” What does this mean? They do not have an effect on the organism.
3.
Which type of substitution mutation completely stops the translation of an mRNA molecule? Nonsense point mutations.
4.
Why are insertion and deletions known as “frameshift mutations?” They alter the “reading” frame of the ribosome from the mutation on through the rest of the sequence.
5.
Mutations will be passed on to future generations only if they occur in which type of cell? GAMETES!!!
